ENGINEERING OF LIVING CELLS FOR THE EXPRESSION OF HOLO-PHYCOBILIPROTEIN-BASED CONSTRUCTS

Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precurso r of the fusion protein comprising a corresponding apo-phycobiliprotein dom...

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Bibliographische Detailangaben
Hauptverfasser:	TOOLEY, AARON J, CAI, YUPING, GLAZER, ALEXANDER N
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precurso r of the fusion protein comprising a corresponding apo-phycobiliprotein domain , and a recombinant phycobiliprotein domain-bilin lyase, which components reac t to form the holo-phycobiliprotein fusion protein. Also described are holo- phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo- phycobiliprotein domain.