METHOD FOR GENOME MINING FOR SECRETED PROTEIN GENES

The present invention relates to a method for screening for compounds secret ed by an organism, comprising (a) Raising antibodies against secreted products of a donor organism, (b) providing a gene library from the donor organism, (c) cloning the gene library into a suitable host organism, (d) expre...

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Bibliographische Detailangaben
Hauptverfasser:	JORGENSEN, BIRTHE RAVN, NIELSEN, PREBEN
Format:	Patent
Sprache:	eng ; fre
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for screening for compounds secret ed by an organism, comprising (a) Raising antibodies against secreted products of a donor organism, (b) providing a gene library from the donor organism, (c) cloning the gene library into a suitable host organism, (d) expressing the cloned genes in the host organism, and (e) detecting positive clones, which upon expression of the cloned genes secretes a compound, using the antibodie s of (a) to identify such positive clones. The invention also relates to compounds, nucleotide sequences and microorganisms identifiable by said meth od.