USE OF A NUCLEOTIDE SEQUENCE FOR ENHANCING PROTEIN SYNTHESISAND EXPRESSION OF PROTEINS

The present invention is related to the use of nucleotide sequences substantially similar to the cDNA sequence (SEQ ID NO:2:) obtainable from th e leader sequence (SEQ ID NO:1:) of the Cocksfoot mottle virus (CfMV) which is capable of enhancing protein synthesis and expression of proteins, especiall...

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Hauptverfasser: TEERI, TEEMU, ASPEGREN, ARNO KRISTIAN, SAARMA, MART, MAKINEN, KRISTIINA MARIA
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creator TEERI, TEEMU
ASPEGREN, ARNO KRISTIAN
SAARMA, MART
MAKINEN, KRISTIINA MARIA
description The present invention is related to the use of nucleotide sequences substantially similar to the cDNA sequence (SEQ ID NO:2:) obtainable from th e leader sequence (SEQ ID NO:1:) of the Cocksfoot mottle virus (CfMV) which is capable of enhancing protein synthesis and expression of proteins, especiall y in plants such as cereals. Also disclosed is a method for producing potentia l enhancer elements by selecting 5'UTRs having a capacity of producing hairpin loop structures and preparing substantially similar nucleic acid sequences. In addition a method for enhancing the expression in plants as well as the properties characteristic for the nucleotide sequence which are responsible for the enhanced expression.
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
title USE OF A NUCLEOTIDE SEQUENCE FOR ENHANCING PROTEIN SYNTHESISAND EXPRESSION OF PROTEINS
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