METHOD OF ISOLATING SECRETION SIGNALS IN LACTIC ACID BACTERIA AND NOVEL SECRETION SIGNALS ISOLATED FROM LACTOCOCCUS LACTIS

A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR and the reporter gene is deleted and the DNA molecule comprises a DNA s...

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Hauptverfasser: ISRAELSEN, HANS, BREDMOSE, LARS, RAVN, PETER, MADSEN, SOEREN MICHAEL, ARNAU, JOSE, JOHNSEN, MADS GROENVOLD, VRANG, ASTRID
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creator ISRAELSEN, HANS
BREDMOSE, LARS
RAVN, PETER
MADSEN, SOEREN MICHAEL
ARNAU, JOSE
JOHNSEN, MADS GROENVOLD
VRANG, ASTRID
description A method of identifying nucleotide sequences coding for signal peptides in lactic acid bacteria, using a DNA molecule comprising a transposon including a promoterless reporter gene from which DNA molecule a region between the LR and the reporter gene is deleted and the DNA molecule comprises a DNA sequence coding for a secretion reporter molecule. By deleting the region between the LR and the reporter gene, stop codons in-frame with the secretion reporter molecule is removed which upon transposition permits translational fusions from upstream the LR.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title METHOD OF ISOLATING SECRETION SIGNALS IN LACTIC ACID BACTERIA AND NOVEL SECRETION SIGNALS ISOLATED FROM LACTOCOCCUS LACTIS
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