HIGH TEMPERATURE REVERSE TRANSCRIPTION USING MUTANT DNA POLYMERASES

The present invention relates to improved reverse transcription methods usin g a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.

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Bibliographische Detailangaben
Hauptverfasser: SCHONBRUNNER, NANCY JENEANE, ELFSTROM, CARITA MARIA, MYERS, THOMAS WILLIAM, HIGUCHI, RUSSELL GENE, WANG, ALICE MING, SMITH, EDWARD SOH, GELFAND, DAVID HARROW
Format: Patent
Sprache:eng ; fre
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