USE OF O-.BETA.-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS FOR THE EVALUATION OF INTESTINAL LACTASE,AND PRODUCTION PROCESS

There is disclosed the use of O-.beta.-D-galactopyranosyl-D- xyloses, particularly 2-O-.beta.-D-galactopyranosyl-D-xylose, and 3-O-.beta.-D-galactopyranosyl--D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to...

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Hauptverfasser: CANADA VICINAY, FRANCISCO JAVIER, FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO, MARTIN LOMAS, MANUEL, LOPEZ ALVAREZ, ROSA, VILLANUEVA TORREGROZA, DANIEL, ARAGON REYES, JUAN JOSE
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creator CANADA VICINAY, FRANCISCO JAVIER
FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO
MARTIN LOMAS, MANUEL
LOPEZ ALVAREZ, ROSA
VILLANUEVA TORREGROZA, DANIEL
ARAGON REYES, JUAN JOSE
description There is disclosed the use of O-.beta.-D-galactopyranosyl-D- xyloses, particularly 2-O-.beta.-D-galactopyranosyl-D-xylose, and 3-O-.beta.-D-galactopyranosyl--D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. There is also disclosed a process for producing O-.beta.-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of .beta.-D- galactopyranoside in the presence of an enzyme .beta.- galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37.degree.C; deactivating the .beta.-galactosidase by heating at 100.degree.C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.
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There is also disclosed a process for producing O-.beta.-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of .beta.-D- galactopyranoside in the presence of an enzyme .beta.- galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37.degree.C; deactivating the .beta.-galactosidase by heating at 100.degree.C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.</description><edition>6</edition><language>eng ; fre</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; DERIVATIVES THEREOF ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC CHEMISTRY ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; SUGARS ; VINEGAR ; WINE</subject><creationdate>2002</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20020813&amp;DB=EPODOC&amp;CC=CA&amp;NR=2209927C$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20020813&amp;DB=EPODOC&amp;CC=CA&amp;NR=2209927C$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CANADA VICINAY, FRANCISCO JAVIER</creatorcontrib><creatorcontrib>FERNANDEZ-MAYORALAS ALVAREZ, ALFONSO</creatorcontrib><creatorcontrib>MARTIN LOMAS, MANUEL</creatorcontrib><creatorcontrib>LOPEZ ALVAREZ, ROSA</creatorcontrib><creatorcontrib>VILLANUEVA TORREGROZA, DANIEL</creatorcontrib><creatorcontrib>ARAGON REYES, JUAN JOSE</creatorcontrib><title>USE OF O-.BETA.-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS FOR THE EVALUATION OF INTESTINAL LACTASE,AND PRODUCTION PROCESS</title><description>There is disclosed the use of O-.beta.-D-galactopyranosyl-D- xyloses, particularly 2-O-.beta.-D-galactopyranosyl-D-xylose, and 3-O-.beta.-D-galactopyranosyl--D-xylose, for the preparation of compositions and solutions intended to the evaluation of the intestinal lactase, which present an affinity to the substantially enhanced lactase. 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There is also disclosed a process for producing O-.beta.-D-galactopyranosyl-D-xyloses, comprising: reacting a D-xylose and a substrate of .beta.-D- galactopyranoside in the presence of an enzyme .beta.- galactosidase, in an aqueous medium buffered to a pH comprised between 5.0 and 9.0 and at temperature between 4 and 37.degree.C; deactivating the .beta.-galactosidase by heating at 100.degree.C when reaching the maximum yield of formation of disaccharides detected through thin layer chromatography, and isolating the formed disaccharides through filtration in a packed column with a thinner selected between water and water/alcohol.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record>
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
DERIVATIVES THEREOF
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
SUGARS
VINEGAR
WINE
title USE OF O-.BETA.-D-GALACTOPYRANOSYL-D-XYLOSES FOR THE PREPARATION OF COMPOSITIONS AND SOLUTIONS FOR THE EVALUATION OF INTESTINAL LACTASE,AND PRODUCTION PROCESS
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