RAPID PROPAGATION OF SV3T3 CELLS FOR PRODUCTION OF MIF AND TAF

SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an incolum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree.C while continuing the agitation of the med...

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1. Verfasser: TOLBERT, WILLIAM R
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creator TOLBERT, WILLIAM R
description SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an incolum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30.degree.-38.degree.C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium,whereby said cells are proliferated in aggregated and elaborate HIF and TAF in significantly detectable amounts.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
title RAPID PROPAGATION OF SV3T3 CELLS FOR PRODUCTION OF MIF AND TAF
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