método de uso de alfa-amilase a partir de aspergillus clavatus e de isoamilase para sacarificação

A fungal alpha-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharifi...

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Hauptverfasser: CASPER VROEMEN, MARTIJN SILVAN SCHEFFERS, MARCO VAN BRUSSEL-ZWIJNEN
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creator CASPER VROEMEN
MARTIJN SILVAN SCHEFFERS
MARCO VAN BRUSSEL-ZWIJNEN
description A fungal alpha-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
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subjects ANIMAL AND VEGETABLE OILS, FATS, FATTY SUBSTANCES AND WAXES
BEER
BIOCHEMISTRY
CANDLES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
DETERGENT COMPOSITIONS
DETERGENTS
ENZYMOLOGY
FATTY ACIDS THEREFROM
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
FODDER
FOODS OR FOODSTUFFS
FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J
HUMAN NECESSITIES
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
RECOVERY OF GLYCEROL
RESIN SOAPS
SOAP OR SOAP-MAKING
SPIRITS
THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT
THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
USE OF SINGLE SUBSTANCES AS DETERGENTS
VINEGAR
WINE
title método de uso de alfa-amilase a partir de aspergillus clavatus e de isoamilase para sacarificação
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