Chitinase, dna coding therefor and plants containing same

The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoresis. The invention further provides antifungal compositions comprisin...

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Hauptverfasser: MARION APOTHEKER-DE GROOT, ANNE SILENE PONSTEIN, HUBERTUS JOSEPHUS MARIA LINTHORST, JOHN FERDINAND BOL, LEO SJOERD MELCHERS, MARIANNE BEATRIX SELA-BUURLAGE, BERNARDUS JOHANNES CLEMENS CORNELISSEN
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creator MARION APOTHEKER-DE GROOT
ANNE SILENE PONSTEIN
HUBERTUS JOSEPHUS MARIA LINTHORST
JOHN FERDINAND BOL
LEO SJOERD MELCHERS
MARIANNE BEATRIX SELA-BUURLAGE
BERNARDUS JOHANNES CLEMENS CORNELISSEN
description The present invention provides a novel class of plant chitinases, which have endo-chitinase activity, antifungal activity and a molecular weight of about 40 to 43 kDa as judged by Sodium Dodecyl Sulphate Polyacrylamide electrophoresis. The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention a isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.
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The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention a isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. 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The invention further provides antifungal compositions comprising as active ingredient a chitinase according to the invention. According to a different aspect of the invention a isolated DNA sequence is provided which comprises an open reading frame encoding a novel chitinase. The invention also encompasses a plant expressible recombinant DNA sequence which comprises a DNA sequence encoding a chitinase according to the invention, as well as plant cells and plants which have such recombinant DNA incorporated into their genome.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record>
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
ORGANIC CHEMISTRY
PEPTIDES
PEST REPELLANTS OR ATTRACTANTS
PLANT GROWTH REGULATORS
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
TRAPPING
VINEGAR
WINE
title Chitinase, dna coding therefor and plants containing same
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