Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryoge...
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| creator | ALBERT P. KAUSCH MARY L. MANGANO THOMAS R. ADAMS WHITNEY R. ADAMS JR T. MICHAEL SPENCER WILLIAM G. START THOMAS B. RICE ROGER W. KRUEGER SHERYL A. CHAMBERS WILLIAM J. GORDON-KAMM JAMES V. O'BRIEN RICHARD J. DAINES PEGGY G. LEMAUX CATHERINE J. MACKEY NANCY WILLETTS |
| description | This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen. |
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GORDON-KAMM ; JAMES V. O'BRIEN ; RICHARD J. DAINES ; PEGGY G. LEMAUX ; CATHERINE J. MACKEY ; NANCY WILLETTS</creator><creatorcontrib>ALBERT P. KAUSCH ; MARY L. MANGANO ; THOMAS R. ADAMS ; WHITNEY R. ADAMS JR ; T. MICHAEL SPENCER ; WILLIAM G. START ; THOMAS B. RICE ; ROGER W. KRUEGER ; SHERYL A. CHAMBERS ; WILLIAM J. GORDON-KAMM ; JAMES V. O'BRIEN ; RICHARD J. DAINES ; PEGGY G. LEMAUX ; CATHERINE J. MACKEY ; NANCY WILLETTS</creatorcontrib><description>This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.</description><edition>5</edition><language>eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; ORGANIC CHEMISTRY ; PEPTIDES ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>1993</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19931202&DB=EPODOC&CC=AU&NR=644097B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19931202&DB=EPODOC&CC=AU&NR=644097B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ALBERT P. KAUSCH</creatorcontrib><creatorcontrib>MARY L. MANGANO</creatorcontrib><creatorcontrib>THOMAS R. ADAMS</creatorcontrib><creatorcontrib>WHITNEY R. ADAMS JR</creatorcontrib><creatorcontrib>T. MICHAEL SPENCER</creatorcontrib><creatorcontrib>WILLIAM G. START</creatorcontrib><creatorcontrib>THOMAS B. RICE</creatorcontrib><creatorcontrib>ROGER W. KRUEGER</creatorcontrib><creatorcontrib>SHERYL A. CHAMBERS</creatorcontrib><creatorcontrib>WILLIAM J. GORDON-KAMM</creatorcontrib><creatorcontrib>JAMES V. O'BRIEN</creatorcontrib><creatorcontrib>RICHARD J. DAINES</creatorcontrib><creatorcontrib>PEGGY G. LEMAUX</creatorcontrib><creatorcontrib>CATHERINE J. MACKEY</creatorcontrib><creatorcontrib>NANCY WILLETTS</creatorcontrib><title>Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof</title><description>This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1993</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDEKAjEQANNYiPqHfYCC6KFYeqLY2Gl9rMmGCybZkF0Lf68H9wCrgWGYqXndSHt2ApgdWE6FJWjgLOC5gvYEpbJ728EBexDFZ_yAVszyKxK5JXiqGiJB4syWFUrErOORYpRhU4n93Ew8RqHFyJmBy_l-uq6ocEdS0FIm7Y6PXdOsD_u23Wz_SL6uUUHB</recordid><startdate>19931202</startdate><enddate>19931202</enddate><creator>ALBERT P. KAUSCH</creator><creator>MARY L. MANGANO</creator><creator>THOMAS R. ADAMS</creator><creator>WHITNEY R. ADAMS JR</creator><creator>T. MICHAEL SPENCER</creator><creator>WILLIAM G. START</creator><creator>THOMAS B. RICE</creator><creator>ROGER W. KRUEGER</creator><creator>SHERYL A. CHAMBERS</creator><creator>WILLIAM J. GORDON-KAMM</creator><creator>JAMES V. O'BRIEN</creator><creator>RICHARD J. DAINES</creator><creator>PEGGY G. LEMAUX</creator><creator>CATHERINE J. MACKEY</creator><creator>NANCY WILLETTS</creator><scope>EVB</scope></search><sort><creationdate>19931202</creationdate><title>Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof</title><author>ALBERT P. KAUSCH ; MARY L. MANGANO ; THOMAS R. ADAMS ; WHITNEY R. ADAMS JR ; T. MICHAEL SPENCER ; WILLIAM G. START ; THOMAS B. RICE ; ROGER W. KRUEGER ; SHERYL A. CHAMBERS ; WILLIAM J. GORDON-KAMM ; JAMES V. O'BRIEN ; RICHARD J. DAINES ; PEGGY G. LEMAUX ; CATHERINE J. 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KAUSCH</creatorcontrib><creatorcontrib>MARY L. MANGANO</creatorcontrib><creatorcontrib>THOMAS R. ADAMS</creatorcontrib><creatorcontrib>WHITNEY R. ADAMS JR</creatorcontrib><creatorcontrib>T. MICHAEL SPENCER</creatorcontrib><creatorcontrib>WILLIAM G. START</creatorcontrib><creatorcontrib>THOMAS B. RICE</creatorcontrib><creatorcontrib>ROGER W. KRUEGER</creatorcontrib><creatorcontrib>SHERYL A. CHAMBERS</creatorcontrib><creatorcontrib>WILLIAM J. GORDON-KAMM</creatorcontrib><creatorcontrib>JAMES V. O'BRIEN</creatorcontrib><creatorcontrib>RICHARD J. DAINES</creatorcontrib><creatorcontrib>PEGGY G. LEMAUX</creatorcontrib><creatorcontrib>CATHERINE J. MACKEY</creatorcontrib><creatorcontrib>NANCY WILLETTS</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ALBERT P. KAUSCH</au><au>MARY L. MANGANO</au><au>THOMAS R. ADAMS</au><au>WHITNEY R. ADAMS JR</au><au>T. MICHAEL SPENCER</au><au>WILLIAM G. START</au><au>THOMAS B. RICE</au><au>ROGER W. KRUEGER</au><au>SHERYL A. CHAMBERS</au><au>WILLIAM J. GORDON-KAMM</au><au>JAMES V. O'BRIEN</au><au>RICHARD J. DAINES</au><au>PEGGY G. LEMAUX</au><au>CATHERINE J. MACKEY</au><au>NANCY WILLETTS</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof</title><date>1993-12-02</date><risdate>1993</risdate><abstract>This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.</abstract><edition>5</edition><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM ORGANIC CHEMISTRY PEPTIDES PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
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