Reverse transcription activity from LTiGTbacillus stearothermophilusLT/iGT dna polymerase in the presence of magnesium

The present invention is directed to a thermostable DNA polymerase from Bacillus stearothermophilus for use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the subs...

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Bibliographische Detailangaben
1. Verfasser:	JUDITH E. T. SCHANKE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is directed to a thermostable DNA polymerase from Bacillus stearothermophilus for use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the substantial absence of manganese ions.