Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences

The invention relates to methods for simultaneously or sequentially detecting multiple nucleic acid analytes in a single medium utilizing oligonucleotide hybridization probes coupled to different chemiluminescent labeling reagents. The methods may be used in a heterogeneous, homogeneous or non-homog...

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Bibliographische Detailangaben
Hauptverfasser: NORMAN C. NELSON, JAMES S WOODHEAD, AZZOUZ BEN CHEIKH, IAN WEEKS
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates to methods for simultaneously or sequentially detecting multiple nucleic acid analytes in a single medium utilizing oligonucleotide hybridization probes coupled to different chemiluminescent labeling reagents. The methods may be used in a heterogeneous, homogeneous or non-homogeneous assay system. The invention also relates to specific combinations of chemiluminescent labeling reagents suitable, when coupled to an oligonucleotide probe, for use together in methods for the detection of multiple nucleic acid analytes. The invention also concerns kits useful in these methods.