Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata

A method of producing plants with an increased number of stomata relative to control plants comprises the steps of: (i) inhibiting in plant material the production of fatty acids which stimulate the synthesis of the 14-3-3 class of transcription factors, or otherwise preventing the fatty acids from...

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Hauptverfasser: PETER CHRISTIAAN SIJMONS, GEOFFREY HEYS HOLROYD, JULIE ELIZABETH GRAY, FREDERIQUE MARIANNE VAN DER LEE, ALISTAIR MACULLOCH HETHERINGTON
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creator PETER CHRISTIAAN SIJMONS
GEOFFREY HEYS HOLROYD
JULIE ELIZABETH GRAY
FREDERIQUE MARIANNE VAN DER LEE
ALISTAIR MACULLOCH HETHERINGTON
description A method of producing plants with an increased number of stomata relative to control plants comprises the steps of: (i) inhibiting in plant material the production of fatty acids which stimulate the synthesis of the 14-3-3 class of transcription factors, or otherwise preventing the fatty acids from stimulating the synthesis of the said factors; (ii) selecting the thus inhibited material; and (iii) regenerating the thus selected material into plants. The inhibition may be achieved by sense co-suppression or anti-sense inhibition of an endogenous gene comprising a sequence which is complementary to one which when incubated at a temperature of between 60 and 65 DEG C in 0.3 strength citrate buffered saline containing 0.1 % SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1 % SDS still hybridises with the sequence depicted in SEQ ID No.1. Preferred sequences for use in this method are depicted as SEQ ID No.1 and 2.
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(ii) selecting the thus inhibited material; and (iii) regenerating the thus selected material into plants. The inhibition may be achieved by sense co-suppression or anti-sense inhibition of an endogenous gene comprising a sequence which is complementary to one which when incubated at a temperature of between 60 and 65 DEG C in 0.3 strength citrate buffered saline containing 0.1 % SDS followed by rinsing at the same temperature with 0.3 strength citrate buffered saline containing 0.1 % SDS still hybridises with the sequence depicted in SEQ ID No.1. Preferred sequences for use in this method are depicted as SEQ ID No.1 and 2.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record>
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subjects AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC
GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
ORGANIC CHEMISTRY
PEPTIDES
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE
TRAPPING
VINEGAR
WINE
title Polynucleotide sequences and their use in a method of producing plants with an increased number of stomata
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