PRODUCTION OF MOLECULES BY PERIPLASMIC ENZYMATIC CATALYSIS

A process for producing a compound of interest from an organic substrate, comprising the steps of: a) preparing a reaction mixture comprising - at least two bacteria genetically modified to express, in their periplasmic space, at least one enzyme E1, E2,..., En, preferably a single enzyme, each of said n bacteria expressing an enzyme different from the other bacteria, - an enzyme E1 being capable of catalysing a first reaction R1 using said organic substrate so as to provide a first product P1, and optionally a first coproduct CoP1, - each of the enzymes E2,..., En, being capable of catalysing a reaction R2,..., Rn, using a product or a coproduct obtained by a reaction Rn-1, so as to provide respectively a product P2,..., Pn, and optionally a coproduct CoP2,..., CoPn, - said organic substrate, and - optionally, a cofactor of said organic substrate, a cofactor of said products obtained by reaction Rn-1, referred to as cofactors CoF1, CoF2,..., CoFn; an additional substrate SC of a coproduct obtained by reaction Rn-1, b) - leaving the reaction mixture to react, and c) - separating the biomass from the supernatant and extracting therefrom said compound of interest consisting of one of the products P1, P2,..., Pn.