NUCLEIC ACID LIBRARY SEQUENCING TECHNIQUES WITH ADAPTER DIMER DETECTION
A library sequencing technique with library quality control metrics is described. Sequence data using a sequencing primer that is complementary to a common adapter sequence in fragments of a nucleic acid sequencing library. The sequencing primer excludes a 3' terminal nucleotide of the common a...
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creator | SABOT, Andrea SANMARTIN, Carla RASOLONJATOVO, Isabelle |
description | A library sequencing technique with library quality control metrics is described. Sequence data using a sequencing primer that is complementary to a common adapter sequence in fragments of a nucleic acid sequencing library. The sequencing primer excludes a 3' terminal nucleotide of the common adapter sequence at a junction with a fragment insert. This exclusion avoids a mismatch region in any adapter dimers present in the sequencing library, and the sequence data includes adapter dimer sequence data, which is used to generate the quality control metrics. |
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Sequence data using a sequencing primer that is complementary to a common adapter sequence in fragments of a nucleic acid sequencing library. The sequencing primer excludes a 3' terminal nucleotide of the common adapter sequence at a junction with a fragment insert. This exclusion avoids a mismatch region in any adapter dimers present in the sequencing library, and the sequence data includes adapter dimer sequence data, which is used to generate the quality control metrics.</description><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230928&DB=EPODOC&CC=AU&NR=2022249734A1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230928&DB=EPODOC&CC=AU&NR=2022249734A1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SABOT, Andrea</creatorcontrib><creatorcontrib>SANMARTIN, Carla</creatorcontrib><creatorcontrib>RASOLONJATOVO, Isabelle</creatorcontrib><title>NUCLEIC ACID LIBRARY SEQUENCING TECHNIQUES WITH ADAPTER DIMER DETECTION</title><description>A library sequencing technique with library quality control metrics is described. Sequence data using a sequencing primer that is complementary to a common adapter sequence in fragments of a nucleic acid sequencing library. The sequencing primer excludes a 3' terminal nucleotide of the common adapter sequence at a junction with a fragment insert. 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Sequence data using a sequencing primer that is complementary to a common adapter sequence in fragments of a nucleic acid sequencing library. The sequencing primer excludes a 3' terminal nucleotide of the common adapter sequence at a junction with a fragment insert. This exclusion avoids a mismatch region in any adapter dimers present in the sequencing library, and the sequence data includes adapter dimer sequence data, which is used to generate the quality control metrics.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
title | NUCLEIC ACID LIBRARY SEQUENCING TECHNIQUES WITH ADAPTER DIMER DETECTION |
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