Cell culture medium for cultivating cells, method for cultivating cells, and method for expressing at least one recombinant protein in a cell culture

The invention relates to a cell culture medium for cultivating cells, containing an iron-citrate-diphosphate complex as an iron source. The invention additionally relates to a method for cultivating cells, one or more cells being replicated or maintained in the cell culture medium according to the i...

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Bibliographische Detailangaben
Hauptverfasser: KOBER, Lars, HERRMANN, Sarah
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates to a cell culture medium for cultivating cells, containing an iron-citrate-diphosphate complex as an iron source. The invention additionally relates to a method for cultivating cells, one or more cells being replicated or maintained in the cell culture medium according to the invention in said method, and to a method for expressing at least one recombinant protein in a cell culture, a nucleic acid being introduced into the cell replicated or maintained in the cell culture medium according to the invention in said method, wherein the nucleic acid causes the production of at least one recombinant protein. The cell culture medium according to the invention is advantageous in that the iron source contained therein dissolves very efficiently and rapidly in an aqueous solution (for example a cell culture medium, a cell culture supplement, or water), can be efficiently imported into the interior of the cells, causes an increased cell viability and an increased product titer (also referred to as product concentration) during the production of recombinant proteins, and is very inexpensive.