A long non-coding RNA porcine Lnc-000649 and its application
The invention provides a long non-encoding RNA porcine Lnc-000649 and its application, the cDNA nucleotide sequence corresponding to the long non-encoding RNA porcine Lnc-000649 is shown in SEQ ID NO: 1. The invention also provides a pair of primers which is shown in SEQ ID NO : 2 and SEQ ID NO : 3,...
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| creator | Wu, Junjing Peng, Xianwen Wu, Huayu Dong, Binke Mei, Shuqi Liu, Guisheng Zhao, Haizhong Li, Lianghua Qiao, Mu Zhou, Jiawei Song, Zhongxu Sun, Hua Hu, Hua |
| description | The invention provides a long non-encoding RNA porcine Lnc-000649 and its application, the cDNA nucleotide sequence corresponding to the long non-encoding RNA porcine Lnc-000649 is shown in SEQ ID NO: 1. The invention also provides a pair of primers which is shown in SEQ ID NO : 2 and SEQ ID NO : 3, and a kit for quantitative real-time PCR of porcine Lnc-000649 gene. Meanwhile, a porcine Lnc-000649 gene overexpression vector is also provided in the invention. A new porcine LncRNA, named Lnc-000649, is first identified in the invention, which is significantly down regulated after PRRSV infection. Further research showed that overexpression of the LncRNA could significantly inhibit the proliferation of PRRSV in cells. This provides a new design target for the research and development of new prevention and control drugs for PRRS, and lays a technical foundation for PRRS disease-resistance breeding. |
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The invention also provides a pair of primers which is shown in SEQ ID NO : 2 and SEQ ID NO : 3, and a kit for quantitative real-time PCR of porcine Lnc-000649 gene. Meanwhile, a porcine Lnc-000649 gene overexpression vector is also provided in the invention. A new porcine LncRNA, named Lnc-000649, is first identified in the invention, which is significantly down regulated after PRRSV infection. Further research showed that overexpression of the LncRNA could significantly inhibit the proliferation of PRRSV in cells. 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The invention also provides a pair of primers which is shown in SEQ ID NO : 2 and SEQ ID NO : 3, and a kit for quantitative real-time PCR of porcine Lnc-000649 gene. Meanwhile, a porcine Lnc-000649 gene overexpression vector is also provided in the invention. A new porcine LncRNA, named Lnc-000649, is first identified in the invention, which is significantly down regulated after PRRSV infection. Further research showed that overexpression of the LncRNA could significantly inhibit the proliferation of PRRSV in cells. 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The invention also provides a pair of primers which is shown in SEQ ID NO : 2 and SEQ ID NO : 3, and a kit for quantitative real-time PCR of porcine Lnc-000649 gene. Meanwhile, a porcine Lnc-000649 gene overexpression vector is also provided in the invention. A new porcine LncRNA, named Lnc-000649, is first identified in the invention, which is significantly down regulated after PRRSV infection. Further research showed that overexpression of the LncRNA could significantly inhibit the proliferation of PRRSV in cells. This provides a new design target for the research and development of new prevention and control drugs for PRRS, and lays a technical foundation for PRRS disease-resistance breeding.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | A long non-coding RNA porcine Lnc-000649 and its application |
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