Methods for assaying biological cells in a microfluidic device

Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, gr...

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Bibliographische Detailangaben
Hauptverfasser:	STADLER, Guido K, KIM, Hansohl E, MARKS, Natalie C, PAI, Vincent Haw Tien, MOCCIARO, Annamaria, BEEMILLER, Peter J, VETTERLI, Thomas M, SMITH, Duane, BRONEVETSKY, Yelena, GOODSELL, Amanda L, TENNEY, John A, MCEWEN, Jason M
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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Beschreibung
Zusammenfassung:	Methods are provided for the assay of secreted biomolecules using automated detection and characterization of micro-objects in a microfluidic device. The biomolecules can be secreted by cells, particularly immunological cells, such as T cells. The biomolecules being assayed can include cytokines, growth factors, and the like. Methods are also provided for assaying the cytotoxicity of a cell with respect to another, target cell. Also provided are kits and non-transitory computer-readable media in which programs are stored for causing a system comprising a computer to perform automated methods for detecting secreted biomolecules and/or cytotoxicity in a microfluidic device.