Method and system for automatically counting microbial colonies
The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a...
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| creator | Marcelpoil, Raphael Rodolphe Wiles, Timothy M |
| description | The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth. |
| format | Patent |
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incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.</description><language>eng</language><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY ; BEER ; BIOCHEMISTRY ; CALCULATING ; CHEMISTRY ; COMPUTING ; COUNTING ; ENZYMOLOGY ; HANDLING RECORD CARRIERS ; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PHYSICS ; PRESENTATION OF DATA ; RECOGNITION OF DATA ; RECORD CARRIERS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210204&DB=EPODOC&CC=AU&NR=2016250792B2$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210204&DB=EPODOC&CC=AU&NR=2016250792B2$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Marcelpoil, Raphael Rodolphe</creatorcontrib><creatorcontrib>Wiles, Timothy M</creatorcontrib><title>Method and system for automatically counting microbial colonies</title><description>The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.</description><subject>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CALCULATING</subject><subject>CHEMISTRY</subject><subject>COMPUTING</subject><subject>COUNTING</subject><subject>ENZYMOLOGY</subject><subject>HANDLING RECORD CARRIERS</subject><subject>IMAGE DATA PROCESSING OR GENERATION, IN GENERAL</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PHYSICS</subject><subject>PRESENTATION OF DATA</subject><subject>RECOGNITION OF DATA</subject><subject>RECORD CARRIERS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD3TS3JyE9RSMxLUSiuLC5JzVVIyy9SSCwtyc9NLMlMTszJqVRIzi_NK8nMS1fIzUwuyk_KTMwBCuXk52WmFvMwsKYl5hSn8kJpbgYVN9cQZw_d1IL8-NTigsTk1LzUknjHUCMDQzMjUwNzSyMnJyNjIpUBAPErMtI</recordid><startdate>20210204</startdate><enddate>20210204</enddate><creator>Marcelpoil, Raphael Rodolphe</creator><creator>Wiles, Timothy M</creator><scope>EVB</scope></search><sort><creationdate>20210204</creationdate><title>Method and system for automatically counting microbial colonies</title><author>Marcelpoil, Raphael Rodolphe ; Wiles, Timothy M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_AU2016250792BB23</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>2021</creationdate><topic>APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CALCULATING</topic><topic>CHEMISTRY</topic><topic>COMPUTING</topic><topic>COUNTING</topic><topic>ENZYMOLOGY</topic><topic>HANDLING RECORD CARRIERS</topic><topic>IMAGE DATA PROCESSING OR GENERATION, IN GENERAL</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PHYSICS</topic><topic>PRESENTATION OF DATA</topic><topic>RECOGNITION OF DATA</topic><topic>RECORD CARRIERS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>Marcelpoil, Raphael Rodolphe</creatorcontrib><creatorcontrib>Wiles, Timothy M</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Marcelpoil, Raphael Rodolphe</au><au>Wiles, Timothy M</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Method and system for automatically counting microbial colonies</title><date>2021-02-04</date><risdate>2021</risdate><abstract>The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CALCULATING CHEMISTRY COMPUTING COUNTING ENZYMOLOGY HANDLING RECORD CARRIERS IMAGE DATA PROCESSING OR GENERATION, IN GENERAL INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PRESENTATION OF DATA RECOGNITION OF DATA RECORD CARRIERS SPIRITS TESTING VINEGAR WINE |
| title | Method and system for automatically counting microbial colonies |
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