3D Imaging of Live Cells with Ultraviolet Radiation

A method for 3D imaging of cells in an optical tomography system (11) includes moving a biological object (1) relatively to a microscope objective (18) to present varying angles of view. The biological object (1) is illuminated with radiation having a spectral bandwidth limited to wavelengths betwee...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	RAHN, J. RICHARD, SEIBEL, ERIC J, NELSON, ALAN C, FAUVER, MARK E
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING ANGLES MEASURING AREAS MEASURING IRREGULARITIES OF SURFACES OR CONTOURS MEASURING LENGTH, THICKNESS OR SIMILAR LINEARDIMENSIONS PHYSICS TESTING
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method for 3D imaging of cells in an optical tomography system (11) includes moving a biological object (1) relatively to a microscope objective (18) to present varying angles of view. The biological object (1) is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object (1) and the microscope objective (18) is sensed with a camera (48) from a plurality of differing view angles. A plurality of pseudoprojections of the biological object (1) from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.