SERUMFREIES MEDIUM ZUR HERSTELLUNG VON RETROVIRALEN VEKTOREN

SERUMFREIES MEDIUM ZUR HERSTELLUNG VON RETROVIRALEN VEKTOREN

A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a st...

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Hauptverfasser: FUNAKOSHI, MASAO, YOSHIOKA, HIROFUMI, MATSUMOTO, KAZUYA, KATAYAMA, YASUSHI, MINENO, JUNICHI, TAKESAKO, KAZUTOH, KATO, IKUNOSHIN, TOMURA, DAISUKE
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creator FUNAKOSHI, MASAO
YOSHIOKA, HIROFUMI
MATSUMOTO, KAZUYA
KATAYAMA, YASUSHI
MINENO, JUNICHI
TAKESAKO, KAZUTOH
KATO, IKUNOSHIN
TOMURA, DAISUKE
description A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.
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title SERUMFREIES MEDIUM ZUR HERSTELLUNG VON RETROVIRALEN VEKTOREN
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