MODIFIZIERTE, TEMPERATURSTABILE DNS-POLYMERASEN FÜR SEQUENZBESTIMMUNG

Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are provided. Such modified thermostable DNA polymerases can be prepared by methods of the recombinant DNA technology. Recombinant thermost...

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Bibliographische Detailangaben
Hauptverfasser:	SIGUA, CHRISTOPHER LIM, KALMAN, LISA VIVIAN, MYERS, THOMAS W, GELFAND, DAVID H, REICHERT, FRED LAWRENCE
Format:	Patent
Sprache:	ger
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Modified thermostable DNA polymerases having enhanced efficiency for incorporating unconventional nucleotides such as those labeled with fluorescein family dyes are provided. Such modified thermostable DNA polymerases can be prepared by methods of the recombinant DNA technology. Recombinant thermostable DNA polymerases prepared in accordance with the present invention are advantageous in many in vitro DNA synthesis applications, such as DNA sequencing, synthesis of labeled DNA and the production of labeled primer extension products. The said polymerase enzymes are particularly useful in chain termination nucleic acid sequencing methods. Nucleic acids encoding the recombinant thermostable DNA polymerases of the present invention, as well as vectors and host cells comprising same are also provided. Kits comprising the recombinant thermostable DNA polymerases of the present invention are provided too. The polymerases and methods of the present invention provide significant cost and efficiency advantages for DNA sequencing.