NÄHRMEDIUM FÜR HEFEKULTUR

A new nutritional medium was developed for cultivate yeast, especially intended for selective, simultaneous isolation, differentiation and identification of yeasts, particularly of species of the genus Candida of clinical or environmental relevance. The essential element of the invention lies on the use of a combination of sweet potato extract and yeast or tomato extract to promote growth of diverse fungus species. This nutritional medium incorporates specific chromogenic and/or fluorogenic substrates to detect enzymatic activity of phospholipases, aminopeptidases, hexosaminidases and glucosidases, and substances containing proteins to detect the proteolytic activity pertaining to species of the genus Candida . This medium contains also carbon sources, salts, buffering agents, bacteria inhibitors and agar. This nutritional medium allows differentiation of Candida species by coloration of the colonies, emission of fluorescence under ultraviolet light, formation of halos and morphology of the colonies.