VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN
The invention relates to a process for preparing fibrinogen of high purity. This process is characterised in that it comprises, starting with cryoprecipitates from blood plasma of animal or human origin, placed in aqueous solution, treating this solution so as to bring it to an ionic strength equal...
Gespeichert in:
| Hauptverfasser: | , , , |
|---|---|
| Format: | Patent |
| Sprache: | ger |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | DAZEY, BERNARD HAMSANY, MOHAMED ENFEDAQUE-MORER, SYLVIA VEZON, GERARD |
| description | The invention relates to a process for preparing fibrinogen of high purity. This process is characterised in that it comprises, starting with cryoprecipitates from blood plasma of animal or human origin, placed in aqueous solution, treating this solution so as to bring it to an ionic strength equal to that of a buffer solution having the following composition: NaCl 120 mM Tris 20 mM pH 8.8 this solution, of adjusted ionic strength, is passed through an anion exchange column comprising an anion exchange gel, for example in the form of beads, based on crosslinked agarose comprising quaternary amine groups, especially at the end of a small spacer arm, for example of the C1-C6-alkylene type linked to the agarose beads, in particular containing about 6% agarose and an elution is carried out using a first eluting solution of the same ionic strength, these conditions making it possible to adsorb at least selectively the fibrinogen onto the said column, which fibrinogen is desorbed using an eluting solution of higher ionic strength at 200 mM NaCl. A fibrinogen of high purity is obtained. |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_ATE141284TT1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ATE141284TT1</sourcerecordid><originalsourceid>FETCH-epo_espacenet_ATE141284TT13</originalsourceid><addsrcrecordid>eNrjZDAOcw1yc_QIcvVTiAoNUvBwDQoOcfXxCfVzVwjz91Pw8HcGynn6ufoquHk6BXn6-bu7-vEwsKYl5hSn8kJpbgZFN9cQZw_d1IL8-NTigsTk1LzUknjHEFdDE0MjC5OQEENjYtQAAM8sJ-s</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN</title><source>esp@cenet</source><creator>DAZEY, BERNARD ; HAMSANY, MOHAMED ; ENFEDAQUE-MORER, SYLVIA ; VEZON, GERARD</creator><creatorcontrib>DAZEY, BERNARD ; HAMSANY, MOHAMED ; ENFEDAQUE-MORER, SYLVIA ; VEZON, GERARD</creatorcontrib><description>The invention relates to a process for preparing fibrinogen of high purity. This process is characterised in that it comprises, starting with cryoprecipitates from blood plasma of animal or human origin, placed in aqueous solution, treating this solution so as to bring it to an ionic strength equal to that of a buffer solution having the following composition: NaCl 120 mM Tris 20 mM pH 8.8 this solution, of adjusted ionic strength, is passed through an anion exchange column comprising an anion exchange gel, for example in the form of beads, based on crosslinked agarose comprising quaternary amine groups, especially at the end of a small spacer arm, for example of the C1-C6-alkylene type linked to the agarose beads, in particular containing about 6% agarose and an elution is carried out using a first eluting solution of the same ionic strength, these conditions making it possible to adsorb at least selectively the fibrinogen onto the said column, which fibrinogen is desorbed using an eluting solution of higher ionic strength at 200 mM NaCl. A fibrinogen of high purity is obtained.</description><edition>6</edition><language>ger</language><subject>CHEMISTRY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><creationdate>1996</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960815&DB=EPODOC&CC=AT&NR=E141284T1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19960815&DB=EPODOC&CC=AT&NR=E141284T1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>DAZEY, BERNARD</creatorcontrib><creatorcontrib>HAMSANY, MOHAMED</creatorcontrib><creatorcontrib>ENFEDAQUE-MORER, SYLVIA</creatorcontrib><creatorcontrib>VEZON, GERARD</creatorcontrib><title>VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN</title><description>The invention relates to a process for preparing fibrinogen of high purity. This process is characterised in that it comprises, starting with cryoprecipitates from blood plasma of animal or human origin, placed in aqueous solution, treating this solution so as to bring it to an ionic strength equal to that of a buffer solution having the following composition: NaCl 120 mM Tris 20 mM pH 8.8 this solution, of adjusted ionic strength, is passed through an anion exchange column comprising an anion exchange gel, for example in the form of beads, based on crosslinked agarose comprising quaternary amine groups, especially at the end of a small spacer arm, for example of the C1-C6-alkylene type linked to the agarose beads, in particular containing about 6% agarose and an elution is carried out using a first eluting solution of the same ionic strength, these conditions making it possible to adsorb at least selectively the fibrinogen onto the said column, which fibrinogen is desorbed using an eluting solution of higher ionic strength at 200 mM NaCl. A fibrinogen of high purity is obtained.</description><subject>CHEMISTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1996</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDAOcw1yc_QIcvVTiAoNUvBwDQoOcfXxCfVzVwjz91Pw8HcGynn6ufoquHk6BXn6-bu7-vEwsKYl5hSn8kJpbgZFN9cQZw_d1IL8-NTigsTk1LzUknjHEFdDE0MjC5OQEENjYtQAAM8sJ-s</recordid><startdate>19960815</startdate><enddate>19960815</enddate><creator>DAZEY, BERNARD</creator><creator>HAMSANY, MOHAMED</creator><creator>ENFEDAQUE-MORER, SYLVIA</creator><creator>VEZON, GERARD</creator><scope>EVB</scope></search><sort><creationdate>19960815</creationdate><title>VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN</title><author>DAZEY, BERNARD ; HAMSANY, MOHAMED ; ENFEDAQUE-MORER, SYLVIA ; VEZON, GERARD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_ATE141284TT13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>ger</language><creationdate>1996</creationdate><topic>CHEMISTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><toplevel>online_resources</toplevel><creatorcontrib>DAZEY, BERNARD</creatorcontrib><creatorcontrib>HAMSANY, MOHAMED</creatorcontrib><creatorcontrib>ENFEDAQUE-MORER, SYLVIA</creatorcontrib><creatorcontrib>VEZON, GERARD</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>DAZEY, BERNARD</au><au>HAMSANY, MOHAMED</au><au>ENFEDAQUE-MORER, SYLVIA</au><au>VEZON, GERARD</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN</title><date>1996-08-15</date><risdate>1996</risdate><abstract>The invention relates to a process for preparing fibrinogen of high purity. This process is characterised in that it comprises, starting with cryoprecipitates from blood plasma of animal or human origin, placed in aqueous solution, treating this solution so as to bring it to an ionic strength equal to that of a buffer solution having the following composition: NaCl 120 mM Tris 20 mM pH 8.8 this solution, of adjusted ionic strength, is passed through an anion exchange column comprising an anion exchange gel, for example in the form of beads, based on crosslinked agarose comprising quaternary amine groups, especially at the end of a small spacer arm, for example of the C1-C6-alkylene type linked to the agarose beads, in particular containing about 6% agarose and an elution is carried out using a first eluting solution of the same ionic strength, these conditions making it possible to adsorb at least selectively the fibrinogen onto the said column, which fibrinogen is desorbed using an eluting solution of higher ionic strength at 200 mM NaCl. A fibrinogen of high purity is obtained.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | ger |
| recordid | cdi_epo_espacenet_ATE141284TT1 |
| source | esp@cenet |
| subjects | CHEMISTRY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES |
| title | VERFAHREN ZUR HERSTELLUNG VON HOCHREINEM FIBRINOGEN |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T02%3A35%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=DAZEY,%20BERNARD&rft.date=1996-08-15&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3EATE141284TT1%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |