METODO DE IDENTIFICACION Y CUANTIFICACION DE MICROORGANISMOS UTILES EN LOS PROCESOS DE BIOMINERIA

Método para identificar y cuantificar los microorganismos utiles en procesos de biominería que comprende las etapas de: (a) extraer DNA de la muestra, (b) cuantificar el DNA extraído; (c) opcionalmente realizar al menos un PCR primario con partidores universales para los dominios: i) Bacteria y/o ii) Archaea, para amplificar una region del genoma, (d) ejecutar la técnica PCR cuantitativo (qPCR), empleando como templado la muestra de DNA o en caso dado el producto amplificado obtenido en el PCR primario correspondiente, y partidores específicos para cada taxon a determinar, donde los taxones se escogen entre: i) Bacterias: Bacterias totales, Acidiphilium sp, Leptospirillum sp, Sulfobacillus sp, Acidithiobacillus ferrooxidans y Acidithiobacillus thiooxidans; y ii) Archaeas: Archaeas totales, Acidianus sp, Ferroplasma sp, Metallosphaera sp, Sulfolobus sp, y Thermoplasma sp, calcular el numero de microorganismos de cada uno de los taxones analizados presentes en la muestra. Identifying and quantifying the micro-organisms useful in processes of biomining industry, comprises: extracting the DNA from sample; quantifying the extracted DNA; optionally performing a primary polymerization chain reaction (PCR) with universal starters for the domains Bacteria and/or Archaea, to amplify a genome region; performing quantitative PCR (qPCR), while employing the extracted DNA or the amplified product and the specific starters for each taxon to be determined, as sample matrix; and calculating the number of micro-organisms in each of analyzed taxons. Method to identify and quantify the micro-organisms useful in processes of biomining industry, comprises: extracting the DNA from the sample; quantifying the extracted DNA; optionally performing a primary polymerization chain reaction (PCR) with universal starters for the domains Bacteria and/or Archaea, to amplify a genome region; performing quantitative PCR (qPCR), while employing the extracted DNA or the amplified product obtained in the primary PCR and the specific starters for each taxon to be determined, as sample matrix, where the taxons are Total bacteria, Acidiphilium sp., Leptospirillum sp., Sulfobacillus sp., Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans and Total Archeans, Acidianus sp., Ferroplasma sp., Metallosphaera sp., Sulfolobus sp. and Thermoplasma sp.; and calculating the number of micro-organisms in each of analyzed taxons present in the sample.