Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma
Purpose Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed. Design/methodology/approach Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors...
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| Veröffentlicht in: | Sensor review 2021-08, Vol.41 (3), p.229-234 |
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| creator | He, Xudong Yang, GuangYi Yang, E Zhang, Moli Luo, Dan Liu, Jingjian Zhao, Chongnan Chen, Qinhua Ran, Fengying |
| description | Purpose
Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed.
Design/methodology/approach
Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence.
Findings
This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved.
Originality/value
This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors. |
| doi_str_mv | 10.1108/SR-04-2020-0087 |
| format | Article |
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Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed.
Design/methodology/approach
Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence.
Findings
This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved.
Originality/value
This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.</description><identifier>ISSN: 0260-2288</identifier><identifier>EISSN: 1758-6828</identifier><identifier>DOI: 10.1108/SR-04-2020-0087</identifier><language>eng</language><publisher>Bradford: Emerald Publishing Limited</publisher><subject>Biomarkers ; Biosensors ; Blood plasma ; Electron microscopes ; Enzymes ; Fluorescent indicators ; Graphene ; Lung cancer ; Medical prognosis ; Microspheres ; Nanomaterials ; Nanoparticles ; Plasma ; Quantum dots ; Transmission electron microscopy ; Tumors</subject><ispartof>Sensor review, 2021-08, Vol.41 (3), p.229-234</ispartof><rights>Xudong He, GuangYi Yang, E. Yang, Moli Zhang, Dan Luo, Jingjian Liu, Chongnan Zhao, Qinhua Chen and Fengying Ran.</rights><rights>Xudong He, GuangYi Yang, E. Yang, Moli Zhang, Dan Luo, Jingjian Liu, Chongnan Zhao, Qinhua Chen and Fengying Ran. This work is published under https://creativecommons.org/licenses/by-nc/3.0/legalcode (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-2d4dca9bac1ea6f41325af3da3db9b19c3826a9464be84e62fd77840d373526e3</citedby><cites>FETCH-LOGICAL-c349t-2d4dca9bac1ea6f41325af3da3db9b19c3826a9464be84e62fd77840d373526e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.emerald.com/insight/content/doi/10.1108/SR-04-2020-0087/full/html$$EHTML$$P50$$Gemerald$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,967,11635,27924,27925,52689</link.rule.ids></links><search><creatorcontrib>He, Xudong</creatorcontrib><creatorcontrib>Yang, GuangYi</creatorcontrib><creatorcontrib>Yang, E</creatorcontrib><creatorcontrib>Zhang, Moli</creatorcontrib><creatorcontrib>Luo, Dan</creatorcontrib><creatorcontrib>Liu, Jingjian</creatorcontrib><creatorcontrib>Zhao, Chongnan</creatorcontrib><creatorcontrib>Chen, Qinhua</creatorcontrib><creatorcontrib>Ran, Fengying</creatorcontrib><title>Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma</title><title>Sensor review</title><description>Purpose
Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed.
Design/methodology/approach
Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence.
Findings
This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved.
Originality/value
This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.</description><subject>Biomarkers</subject><subject>Biosensors</subject><subject>Blood plasma</subject><subject>Electron microscopes</subject><subject>Enzymes</subject><subject>Fluorescent indicators</subject><subject>Graphene</subject><subject>Lung cancer</subject><subject>Medical prognosis</subject><subject>Microspheres</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Plasma</subject><subject>Quantum dots</subject><subject>Transmission electron microscopy</subject><subject>Tumors</subject><issn>0260-2288</issn><issn>1758-6828</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>XDTOA</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptkEtLAzEUhYMoWB9rtwHXafOamcxSWh-FomJ1HW4nd2rKTGZMWlB_vVPqRnB1z-J858JHyJXgYyG4mSxfGNdMcskZ56Y4IiNRZIblRppjMuIy50xKY07JWUobzoXUuRqR-m3rG__tw5rOHiEhnVMIjq4j9O8YkHaf3iFtO-drj462sA649RUNELoe4hAbTLTuIk0Y0n7mecYWgvpA33ctBNo3kFq4ICc1NAkvf-85ebu7fZ0-sMXT_Xx6s2CV0uWWSaddBeUKKoGQ11oomUGtHCi3KleirJSROZQ61ys0GnNZu6IwmjtVqEzmqM7J9WG3j93HDtPWbrpdDMNLK7PMqGEx40NrcmhVsUspYm376FuIX1Zwu5dply-Wa7uXafcyB2J8ILDFCI37B_hjX_0Ae6109w</recordid><startdate>20210810</startdate><enddate>20210810</enddate><creator>He, Xudong</creator><creator>Yang, GuangYi</creator><creator>Yang, E</creator><creator>Zhang, Moli</creator><creator>Luo, Dan</creator><creator>Liu, Jingjian</creator><creator>Zhao, Chongnan</creator><creator>Chen, Qinhua</creator><creator>Ran, Fengying</creator><general>Emerald Publishing Limited</general><general>Emerald Group Publishing Limited</general><scope>XDTOA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0U~</scope><scope>1-H</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>7WY</scope><scope>7WZ</scope><scope>7XB</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>F~G</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K6~</scope><scope>L.-</scope><scope>L.0</scope><scope>L6V</scope><scope>L7M</scope><scope>M0C</scope><scope>M2P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>PQBIZ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>S0W</scope></search><sort><creationdate>20210810</creationdate><title>Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma</title><author>He, Xudong ; Yang, GuangYi ; Yang, E ; Zhang, Moli ; Luo, Dan ; Liu, Jingjian ; Zhao, Chongnan ; Chen, Qinhua ; Ran, Fengying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-2d4dca9bac1ea6f41325af3da3db9b19c3826a9464be84e62fd77840d373526e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Biomarkers</topic><topic>Biosensors</topic><topic>Blood plasma</topic><topic>Electron microscopes</topic><topic>Enzymes</topic><topic>Fluorescent indicators</topic><topic>Graphene</topic><topic>Lung cancer</topic><topic>Medical prognosis</topic><topic>Microspheres</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Plasma</topic><topic>Quantum dots</topic><topic>Transmission electron microscopy</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Xudong</creatorcontrib><creatorcontrib>Yang, GuangYi</creatorcontrib><creatorcontrib>Yang, E</creatorcontrib><creatorcontrib>Zhang, Moli</creatorcontrib><creatorcontrib>Luo, Dan</creatorcontrib><creatorcontrib>Liu, Jingjian</creatorcontrib><creatorcontrib>Zhao, Chongnan</creatorcontrib><creatorcontrib>Chen, Qinhua</creatorcontrib><creatorcontrib>Ran, Fengying</creatorcontrib><collection>Emerald Open Access</collection><collection>CrossRef</collection><collection>Global News & ABI/Inform Professional</collection><collection>Trade PRO</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ABI/INFORM Professional Standard</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ABI/INFORM Global</collection><collection>Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>ProQuest One Business</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>DELNET Engineering & Technology Collection</collection><jtitle>Sensor review</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Xudong</au><au>Yang, GuangYi</au><au>Yang, E</au><au>Zhang, Moli</au><au>Luo, Dan</au><au>Liu, Jingjian</au><au>Zhao, Chongnan</au><au>Chen, Qinhua</au><au>Ran, Fengying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma</atitle><jtitle>Sensor review</jtitle><date>2021-08-10</date><risdate>2021</risdate><volume>41</volume><issue>3</issue><spage>229</spage><epage>234</epage><pages>229-234</pages><issn>0260-2288</issn><eissn>1758-6828</eissn><abstract>Purpose
Based on DNase I and reduced graphene oxide (rGO)-magnetic silicon microspheres (MNPS), a highly sensitive and selective fluorescent probe for the detection of PD-L1 was developed.
Design/methodology/approach
Here °C we present a feasibility of biosensor to detection of PD-L1 in lung tumors plasma. In the absence of PD-L1°C the PD-L1 aptamer is absorbed on the surface of graphene oxide modified magnetic nanoparticles °8rGO-MNPS°9 and leading to effective fluorescence quenching. Upon adding PD-L1°C the aptamer sequences could be specifically recognized by PD-L1 and the aptamer/PD-L1 complex is formed°C resulting in the recovery of quenched fluorescence.
Findings
This sensor can detect PD-L1 with a linear range from 100 pg mL−1 to 100 ng mL−1, and a detection limit of 10 pg•m−1 was achieved.
Originality/value
This method provides an easy and sensitive method for the detection of PD-L1 and will be beneficial to the early diagnosis and prognosis of tumors.</abstract><cop>Bradford</cop><pub>Emerald Publishing Limited</pub><doi>10.1108/SR-04-2020-0087</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Sensor review, 2021-08, Vol.41 (3), p.229-234 |
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| language | eng |
| recordid | cdi_emerald_primary_10_1108_SR-04-2020-0087 |
| source | Emerald A-Z Current Journals |
| subjects | Biomarkers Biosensors Blood plasma Electron microscopes Enzymes Fluorescent indicators Graphene Lung cancer Medical prognosis Microspheres Nanomaterials Nanoparticles Plasma Quantum dots Transmission electron microscopy Tumors |
| title | Utilizing DNase I and graphene oxide modified magnetic nanoparticles for sensing PD-L1 in human plasma |
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