Experimental evaluation of doxorubicin drug as anticancer agent using a natural component : an in virto study
HCC is the sixth commonest cancer worldwide and the third one to cause death. In Egypt, liver cancer forms 1.68% of total malignancies. HCC constitutes 70.48% of liver tumors. HCC represents the main complication of cirrhosis. Chemotherapy has an undesirable side effect, so the drugs are used instea...
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| Veröffentlicht in: | Journal of the Egyptian Society of Parasitology 2018, Vol.48 (3), p.699-712 |
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| container_title | Journal of the Egyptian Society of Parasitology |
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| creator | Wahba, Isra M. Muhsin, Riham al-Fiki, Abir A. Muhammad, Ali F. |
| description | HCC is the sixth commonest cancer worldwide and the third one to cause death. In Egypt, liver
cancer forms 1.68% of total malignancies. HCC constitutes 70.48% of liver tumors. HCC represents
the main complication of cirrhosis. Chemotherapy has an undesirable side effect, so the
drugs are used instead. Bee venom and curcumin have been used as folk medicine since ancient
times. This study examined the anticancer potential of bee (Apis mellifera) venom and curcumin
compared with a synthetic anticancer of Doxorubicin (Dox) drug, and tested the effect of combination
between the tested three components to decrease the chemotherapy side effect. Cytotoxicity
activity of Bee venom and curcumin were determined using MTT assay. Cell cycle was
determined by flow cytometry and apoptotic detection by using Annexin V-FITC Assay. RNA
was extracted to evaluate the expression of P53, Bcl2 & NF-κB genes using semi-quantitative
RT-PCR after 24hrs. HepG2 cell line was more save to test bee venom and curcumin onHepG2
cell line. Curcumin and bee venom had IC50 as 15.15 & 31μg/ml with HepG2 cell line. Combination
between Bee venom, curcumin, and doxorubicin drug had apoptotic effect against
HepG2cell line through the elevation by increasing the level of p53 & NF-κB genes and decrease
the Bcl-2 gene expression. |
| format | Article |
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cancer forms 1.68% of total malignancies. HCC constitutes 70.48% of liver tumors. HCC represents
the main complication of cirrhosis. Chemotherapy has an undesirable side effect, so the
drugs are used instead. Bee venom and curcumin have been used as folk medicine since ancient
times. This study examined the anticancer potential of bee (Apis mellifera) venom and curcumin
compared with a synthetic anticancer of Doxorubicin (Dox) drug, and tested the effect of combination
between the tested three components to decrease the chemotherapy side effect. Cytotoxicity
activity of Bee venom and curcumin were determined using MTT assay. Cell cycle was
determined by flow cytometry and apoptotic detection by using Annexin V-FITC Assay. RNA
was extracted to evaluate the expression of P53, Bcl2 & NF-κB genes using semi-quantitative
RT-PCR after 24hrs. HepG2 cell line was more save to test bee venom and curcumin onHepG2
cell line. Curcumin and bee venom had IC50 as 15.15 & 31μg/ml with HepG2 cell line. Combination
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cancer forms 1.68% of total malignancies. HCC constitutes 70.48% of liver tumors. HCC represents
the main complication of cirrhosis. Chemotherapy has an undesirable side effect, so the
drugs are used instead. Bee venom and curcumin have been used as folk medicine since ancient
times. This study examined the anticancer potential of bee (Apis mellifera) venom and curcumin
compared with a synthetic anticancer of Doxorubicin (Dox) drug, and tested the effect of combination
between the tested three components to decrease the chemotherapy side effect. Cytotoxicity
activity of Bee venom and curcumin were determined using MTT assay. Cell cycle was
determined by flow cytometry and apoptotic detection by using Annexin V-FITC Assay. RNA
was extracted to evaluate the expression of P53, Bcl2 & NF-κB genes using semi-quantitative
RT-PCR after 24hrs. HepG2 cell line was more save to test bee venom and curcumin onHepG2
cell line. Curcumin and bee venom had IC50 as 15.15 & 31μg/ml with HepG2 cell line. Combination
between Bee venom, curcumin, and doxorubicin drug had apoptotic effect against
HepG2cell line through the elevation by increasing the level of p53 & NF-κB genes and decrease
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cancer forms 1.68% of total malignancies. HCC constitutes 70.48% of liver tumors. HCC represents
the main complication of cirrhosis. Chemotherapy has an undesirable side effect, so the
drugs are used instead. Bee venom and curcumin have been used as folk medicine since ancient
times. This study examined the anticancer potential of bee (Apis mellifera) venom and curcumin
compared with a synthetic anticancer of Doxorubicin (Dox) drug, and tested the effect of combination
between the tested three components to decrease the chemotherapy side effect. Cytotoxicity
activity of Bee venom and curcumin were determined using MTT assay. Cell cycle was
determined by flow cytometry and apoptotic detection by using Annexin V-FITC Assay. RNA
was extracted to evaluate the expression of P53, Bcl2 & NF-κB genes using semi-quantitative
RT-PCR after 24hrs. HepG2 cell line was more save to test bee venom and curcumin onHepG2
cell line. Curcumin and bee venom had IC50 as 15.15 & 31μg/ml with HepG2 cell line. Combination
between Bee venom, curcumin, and doxorubicin drug had apoptotic effect against
HepG2cell line through the elevation by increasing the level of p53 & NF-κB genes and decrease
the Bcl-2 gene expression.</abstract><cop>Cairo, Egypt</cop><pub>The Egyptian Society of Parasitology</pub><tpages>14</tpages></addata></record> |
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| ispartof | Journal of the Egyptian Society of Parasitology, 2018, Vol.48 (3), p.699-712 |
| issn | 1110-0583 2090-2549 |
| language | eng |
| recordid | cdi_emarefa_primary_901304 |
| source | Alma/SFX Local Collection |
| subjects | الأدوية الأدوية المضادة للأورام السرطان العلاج المناعي |
| title | Experimental evaluation of doxorubicin drug as anticancer agent using a natural component : an in virto study |
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