Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene
One hundred and eight of soil samples have been collected from 17 area loci in Maysan governorate during the period from September to the October 2012 ,and cultivated on lauria agar media for isolation and identification of B. licheniformis. Primary identification was depended on colonialsmorphology...
Gespeichert in:
| Veröffentlicht in: | Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ 2018, Vol.10 (2), p.157-167 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 167 |
|---|---|
| container_issue | 2 |
| container_start_page | 157 |
| container_title | Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ |
| container_volume | 10 |
| creator | Banun, Shayma R. Ali, Zahrah Muhammad |
| description | One hundred and eight of soil samples have been collected from 17 area loci in
Maysan governorate during the period from September to the October 2012 ,and cultivated on
lauria agar media for isolation and identification of B. licheniformis. Primary identification
was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then
the isolates was identified by VITEK-2 compact system Version 05.01 using BCL
identification cards (BCL-ID). The results revealed that 53(94.64%) of isolates identified as
Bacillus licheniformis which could be classified in classes where the very good identification
class with percent of identification reached to 43.39%, followed by good , acceptable, and
low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as
unidentified organism. The isolates were identified using the PCR technique for detection of
gyrase B gene clarify that 56(100%) isolates of B. licheniformis with gyrase B gene that
encodes the subunit B protein of DNA gyrase |
| format | Article |
| fullrecord | <record><control><sourceid>emarefa</sourceid><recordid>TN_cdi_emarefa_primary_899002</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>899002</sourcerecordid><originalsourceid>FETCH-emarefa_primary_8990023</originalsourceid><addsrcrecordid>eNqFyc2KwjAUhuEwODDieAkD5wYKadNq3Tr4h1txW471xB4mTSQns-jdW8G9q-_hez_UtDB5ni2qspyM1kuT1XVVfqm5CF90oStTmnwxVX-HK_nElltMHDykjsCFFt1TsQ_3jh23ICkie4FgYY0tO_cvMP4debYh9ixwGeB8OG2OWQEySKIe0F9hN0QUgjXcyNO3-rTohOavnamf7eb0u8-ox0gWm3vkUUNTr1ZaF-ZdfwCDcEaC</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Banun, Shayma R. ; Ali, Zahrah Muhammad</creator><creatorcontrib>Banun, Shayma R. ; Ali, Zahrah Muhammad</creatorcontrib><description>One hundred and eight of soil samples have been collected from 17 area loci in
Maysan governorate during the period from September to the October 2012 ,and cultivated on
lauria agar media for isolation and identification of B. licheniformis. Primary identification
was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then
the isolates was identified by VITEK-2 compact system Version 05.01 using BCL
identification cards (BCL-ID). The results revealed that 53(94.64%) of isolates identified as
Bacillus licheniformis which could be classified in classes where the very good identification
class with percent of identification reached to 43.39%, followed by good , acceptable, and
low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as
unidentified organism. The isolates were identified using the PCR technique for detection of
gyrase B gene clarify that 56(100%) isolates of B. licheniformis with gyrase B gene that
encodes the subunit B protein of DNA gyrase</description><identifier>ISSN: 2073-8854</identifier><identifier>EISSN: 2311-6544</identifier><language>eng</language><publisher>Najaf, Iraq: University of Kufa, Faculty of Science, Department of live Sciences</publisher><ispartof>Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ, 2018, Vol.10 (2), p.157-167</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Banun, Shayma R.</creatorcontrib><creatorcontrib>Ali, Zahrah Muhammad</creatorcontrib><title>Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene</title><title>Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ</title><description>One hundred and eight of soil samples have been collected from 17 area loci in
Maysan governorate during the period from September to the October 2012 ,and cultivated on
lauria agar media for isolation and identification of B. licheniformis. Primary identification
was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then
the isolates was identified by VITEK-2 compact system Version 05.01 using BCL
identification cards (BCL-ID). The results revealed that 53(94.64%) of isolates identified as
Bacillus licheniformis which could be classified in classes where the very good identification
class with percent of identification reached to 43.39%, followed by good , acceptable, and
low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as
unidentified organism. The isolates were identified using the PCR technique for detection of
gyrase B gene clarify that 56(100%) isolates of B. licheniformis with gyrase B gene that
encodes the subunit B protein of DNA gyrase</description><issn>2073-8854</issn><issn>2311-6544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFyc2KwjAUhuEwODDieAkD5wYKadNq3Tr4h1txW471xB4mTSQns-jdW8G9q-_hez_UtDB5ni2qspyM1kuT1XVVfqm5CF90oStTmnwxVX-HK_nElltMHDykjsCFFt1TsQ_3jh23ICkie4FgYY0tO_cvMP4debYh9ixwGeB8OG2OWQEySKIe0F9hN0QUgjXcyNO3-rTohOavnamf7eb0u8-ox0gWm3vkUUNTr1ZaF-ZdfwCDcEaC</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Banun, Shayma R.</creator><creator>Ali, Zahrah Muhammad</creator><general>University of Kufa, Faculty of Science, Department of live Sciences</general><scope>ADJCN</scope><scope>AHFXO</scope></search><sort><creationdate>2018</creationdate><title>Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene</title><author>Banun, Shayma R. ; Ali, Zahrah Muhammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-emarefa_primary_8990023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>online_resources</toplevel><creatorcontrib>Banun, Shayma R.</creatorcontrib><creatorcontrib>Ali, Zahrah Muhammad</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><jtitle>Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Banun, Shayma R.</au><au>Ali, Zahrah Muhammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene</atitle><jtitle>Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ</jtitle><date>2018</date><risdate>2018</risdate><volume>10</volume><issue>2</issue><spage>157</spage><epage>167</epage><pages>157-167</pages><issn>2073-8854</issn><eissn>2311-6544</eissn><abstract>One hundred and eight of soil samples have been collected from 17 area loci in
Maysan governorate during the period from September to the October 2012 ,and cultivated on
lauria agar media for isolation and identification of B. licheniformis. Primary identification
was depended on colonialsmorphology, microscopicsexamination and biochemicalstests then
the isolates was identified by VITEK-2 compact system Version 05.01 using BCL
identification cards (BCL-ID). The results revealed that 53(94.64%) of isolates identified as
Bacillus licheniformis which could be classified in classes where the very good identification
class with percent of identification reached to 43.39%, followed by good , acceptable, and
low discrimination , which were 30.18% ,24.52% and 1.88% respectively and 3(5.35%) as
unidentified organism. The isolates were identified using the PCR technique for detection of
gyrase B gene clarify that 56(100%) isolates of B. licheniformis with gyrase B gene that
encodes the subunit B protein of DNA gyrase</abstract><cop>Najaf, Iraq</cop><pub>University of Kufa, Faculty of Science, Department of live Sciences</pub><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2073-8854 |
| ispartof | Mağallaẗ ğāmiʿaẗ al-kūfaẗ li-ʿulūm al-ḥayāẗ, 2018, Vol.10 (2), p.157-167 |
| issn | 2073-8854 2311-6544 |
| language | eng |
| recordid | cdi_emarefa_primary_899002 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| title | Identification the local thermophilic strains of Bacillus licheniformis by VITEK-2 system and Gyrase B gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-16T09%3A38%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-emarefa&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20the%20local%20thermophilic%20strains%20of%20Bacillus%20licheniformis%20by%20VITEK-2%20system%20and%20Gyrase%20B%20gene&rft.jtitle=Ma%C4%9Falla%E1%BA%97%20%C4%9F%C4%81mi%CA%BFa%E1%BA%97%20al-k%C5%ABfa%E1%BA%97%20li-%CA%BFul%C5%ABm%20al-%E1%B8%A5ay%C4%81%E1%BA%97&rft.au=Banun,%20Shayma%20R.&rft.date=2018&rft.volume=10&rft.issue=2&rft.spage=157&rft.epage=167&rft.pages=157-167&rft.issn=2073-8854&rft.eissn=2311-6544&rft_id=info:doi/&rft_dat=%3Cemarefa%3E899002%3C/emarefa%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |