Potential healing effect of topical stem cell transplantation and methandrostenoloneon in induced cutaneous wounds in dogs

This study was done to explore the influence of stem cells on cutaneous wounds in comparison with treatment with methandrostenoloneon drug. Cutaneous wound was induced on the back of each dog using disposable dermal biopsy punches . Twenty five dogs were divided into five groups: 1stgroup (T1) was control. 2nd group (T2) was induced without any treatment. 3rd group (T3) was induced wound and treated with phosphate buffer saline, 4rth group (T4) was induced and treated with 5mg/kg/B.W. of Methandrostenolone, 5th group (T5) was induced and treated with stem cells 1x106cell/250 μL of phosphate buffer saline. After 7 and 15 days post treatment level of Hb and RBCs count in T2 and T3 group were decline as compared with control group. The WBCs, Alkaline phosphatase , acid phosphataseas in T2 and T3 showed enhance as contrast with T1. After 7 days, Hb and RBCs in T4 and T5 showed important increase as compared with T2. Moreover the WBCs, alkaline phosphatase, acid phosphatase and total protein levels significantly elevated in the T4 and T5 as compared with T1. After 15 days of treatment the Hb, RBCs, levels of Alkaline phosphatase, acid phosphataseas and total protein levels in T4 showed significant increase as compared with T1, The Hb, RBCs, levels of Alkaline phosphatase, acid phosphataseas and total protein in T5 showed no significant changes as compared with control group (T1). The histopathological section in (T2) explained less collagen and more inflammatory cells, the section of wound tissue in (T4) appeared incomplete healing wound tissue and presence little inflammatory cells, the histopathological section of wound treated in (T5) showed return the wound to normal tissue. From this result it can be concluded that the MSCs have ability to access healing of the wound in comparison with methandrostenolone without any side effect.