Detection of Giardia Intestinalis Coproantigens in Diarrheic Samples by Immunochromatographic and Elisatechniques
-aimed to search for specific and sensitive diagnostic tests to avoid loss of infected cases with Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through comparison between direct parasitological method, an enzyme linked immunosorbent assay (ELISA) and immunoc...
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| Veröffentlicht in: | Journal of the Egyptian Society of Parasitology 2015-08, Vol.45 (2), p.273-283 |
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| container_title | Journal of the Egyptian Society of Parasitology |
| container_volume | 45 |
| creator | Taha , Afaf Abd El-Raouf Abd El-Aal , Naglaa Fathy Farag , Tahani Ismail Yousef , Asmaa Mohammed Selim , Monira Abd El- Wahab |
| description | -aimed to search for specific and sensitive diagnostic tests to avoid loss of infected cases with
Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through
comparison between direct parasitological method, an enzyme linked immunosorbent assay
(ELISA) and immunochromatographic test (ICT). A comparative cross-sectional study including
75 cases suffering from diarrhea and other gastrointestinal symptoms suggestive of intest inal
giardiasis as abdominal distention, abdominal pain, anorexia, nausea, vomiting and weight
loss, and 25 cases were without any clinical manifestations enrolled in this study. For every
case, complete history taking and full clinical examination were done. Stool samples were collected
from all cases and investigated by direct parasitological method, ELISA, and immunochromatographic
techniques.
The results showed that the sensitivity of immunochromatographic technique was 96% and
specificity was 96% while sensitivity of ELISA was 98 % and specificity was 96% on comparing
their results to the microscopic examination of stool samples for Giardia intestinalis |
| doi_str_mv | 10.12816/0017571 |
| format | Article |
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Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through
comparison between direct parasitological method, an enzyme linked immunosorbent assay
(ELISA) and immunochromatographic test (ICT). A comparative cross-sectional study including
75 cases suffering from diarrhea and other gastrointestinal symptoms suggestive of intest inal
giardiasis as abdominal distention, abdominal pain, anorexia, nausea, vomiting and weight
loss, and 25 cases were without any clinical manifestations enrolled in this study. For every
case, complete history taking and full clinical examination were done. Stool samples were collected
from all cases and investigated by direct parasitological method, ELISA, and immunochromatographic
techniques.
The results showed that the sensitivity of immunochromatographic technique was 96% and
specificity was 96% while sensitivity of ELISA was 98 % and specificity was 96% on comparing
their results to the microscopic examination of stool samples for Giardia intestinalis</description><identifier>ISSN: 1110-0583</identifier><identifier>EISSN: 2090-2549</identifier><identifier>DOI: 10.12816/0017571</identifier><identifier>PMID: 26485845</identifier><language>eng</language><publisher>Cairo - Egypt: The Egyptian Society of Parasitology</publisher><subject>Adolescent ; Adult ; Antigens, Protozoan - isolation & purification ; Child ; Child, Preschool ; Diagnosis ; Diarrhea ; DIARRHOEAL DISEASES ; EGYPT ; Enzyme-Linked Immunosorbent Assay - veterinary ; Etiology ; Feces - chemistry ; Female ; GASTROINTESTINAL DISEASES ; GIARDIA ; Giardia lamblia - immunology ; Giardiasis ; Giardiasis - diagnosis ; Humans ; Immunochromatography - methods ; IMMUNODIAGNOSIS ; IMMUNOLOGICAL TECHNIQUES ; Infant ; Male ; Middle Aged ; Young Adult ; أمراض الإسهال ; أمراض المعدة و الأمعاء ; الإسهال ; التشخيص ; التشخيص المناعي ; الجياردية ; تقنيات التحصين ; علم الأمراض ; مسببات الأمراض ; مصر</subject><ispartof>Journal of the Egyptian Society of Parasitology, 2015-08, Vol.45 (2), p.273-283</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://static.almanhal.com/covers/titl/73926/cover-lg.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26485845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taha , Afaf Abd El-Raouf</creatorcontrib><creatorcontrib>Abd El-Aal , Naglaa Fathy</creatorcontrib><creatorcontrib>Farag , Tahani Ismail</creatorcontrib><creatorcontrib>Yousef , Asmaa Mohammed</creatorcontrib><creatorcontrib>Selim , Monira Abd El- Wahab</creatorcontrib><title>Detection of Giardia Intestinalis Coproantigens in Diarrheic Samples by Immunochromatographic and Elisatechniques</title><title>Journal of the Egyptian Society of Parasitology</title><addtitle>J Egypt Soc Parasitol</addtitle><description>-aimed to search for specific and sensitive diagnostic tests to avoid loss of infected cases with
Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through
comparison between direct parasitological method, an enzyme linked immunosorbent assay
(ELISA) and immunochromatographic test (ICT). A comparative cross-sectional study including
75 cases suffering from diarrhea and other gastrointestinal symptoms suggestive of intest inal
giardiasis as abdominal distention, abdominal pain, anorexia, nausea, vomiting and weight
loss, and 25 cases were without any clinical manifestations enrolled in this study. For every
case, complete history taking and full clinical examination were done. Stool samples were collected
from all cases and investigated by direct parasitological method, ELISA, and immunochromatographic
techniques.
The results showed that the sensitivity of immunochromatographic technique was 96% and
specificity was 96% while sensitivity of ELISA was 98 % and specificity was 96% on comparing
their results to the microscopic examination of stool samples for Giardia intestinalis</description><subject>Adolescent</subject><subject>Adult</subject><subject>Antigens, Protozoan - isolation & purification</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Diagnosis</subject><subject>Diarrhea</subject><subject>DIARRHOEAL DISEASES</subject><subject>EGYPT</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Etiology</subject><subject>Feces - chemistry</subject><subject>Female</subject><subject>GASTROINTESTINAL DISEASES</subject><subject>GIARDIA</subject><subject>Giardia lamblia - immunology</subject><subject>Giardiasis</subject><subject>Giardiasis - diagnosis</subject><subject>Humans</subject><subject>Immunochromatography - methods</subject><subject>IMMUNODIAGNOSIS</subject><subject>IMMUNOLOGICAL TECHNIQUES</subject><subject>Infant</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Young Adult</subject><subject>أمراض الإسهال</subject><subject>أمراض المعدة و الأمعاء</subject><subject>الإسهال</subject><subject>التشخيص</subject><subject>التشخيص المناعي</subject><subject>الجياردية</subject><subject>تقنيات التحصين</subject><subject>علم الأمراض</subject><subject>مسببات الأمراض</subject><subject>مصر</subject><issn>1110-0583</issn><issn>2090-2549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpN0E1LxDAQBuAgii7rgn9AydFLNZ9NepT1a0HwoJ7LNE1tpE3WpHvw3xt1XT0MCcnDzPAidELJBWWalpeEUCUV3UMzRipSMCmqfTSjlJKCSM2P0CKlN5JVyUWuQ3TESqGlFnKG3q_tZM3kgsehw3cOYusAr_xk0-Q8DC7hZVjHAH5yr9Yn7Dy-zir21hn8BON6sAk3H3g1jhsfTB_DCFN4jbDuMwDf4pvcBPKQ3rv3jU3H6KCDIdnF9pyjl9ub5-V98fB4t1pePRTACJ-KhkPTKCIMsaqhXBiWr0QbLTrZAmslzWUUVVQTIzrecV2RyigBjBmhGz5H5z998_Zfc6d6dMnYYQBvwybVVDEpmWBM_lETQ0rRdvU6uhHiR01J_Z1xvc0407Nt100z2nYHfxPN4PQH2PxuO9gJWZVl-e8fhhF8D8MOKF6xkn8CB9CKvg</recordid><startdate>20150801</startdate><enddate>20150801</enddate><creator>Taha , Afaf Abd El-Raouf</creator><creator>Abd El-Aal , Naglaa Fathy</creator><creator>Farag , Tahani Ismail</creator><creator>Yousef , Asmaa Mohammed</creator><creator>Selim , Monira Abd El- Wahab</creator><general>The Egyptian Society of Parasitology</general><scope>~6Z</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150801</creationdate><title>Detection of Giardia Intestinalis Coproantigens in Diarrheic Samples by Immunochromatographic and Elisatechniques</title><author>Taha , Afaf Abd El-Raouf ; 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Giardia intestinalis by detection of G. intestinalis coproantigens in diarrheic samples through
comparison between direct parasitological method, an enzyme linked immunosorbent assay
(ELISA) and immunochromatographic test (ICT). A comparative cross-sectional study including
75 cases suffering from diarrhea and other gastrointestinal symptoms suggestive of intest inal
giardiasis as abdominal distention, abdominal pain, anorexia, nausea, vomiting and weight
loss, and 25 cases were without any clinical manifestations enrolled in this study. For every
case, complete history taking and full clinical examination were done. Stool samples were collected
from all cases and investigated by direct parasitological method, ELISA, and immunochromatographic
techniques.
The results showed that the sensitivity of immunochromatographic technique was 96% and
specificity was 96% while sensitivity of ELISA was 98 % and specificity was 96% on comparing
their results to the microscopic examination of stool samples for Giardia intestinalis</abstract><cop>Cairo - Egypt</cop><pub>The Egyptian Society of Parasitology</pub><pmid>26485845</pmid><doi>10.12816/0017571</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of the Egyptian Society of Parasitology, 2015-08, Vol.45 (2), p.273-283 |
| issn | 1110-0583 2090-2549 |
| language | eng |
| recordid | cdi_emarefa_primary_596665 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult Antigens, Protozoan - isolation & purification Child Child, Preschool Diagnosis Diarrhea DIARRHOEAL DISEASES EGYPT Enzyme-Linked Immunosorbent Assay - veterinary Etiology Feces - chemistry Female GASTROINTESTINAL DISEASES GIARDIA Giardia lamblia - immunology Giardiasis Giardiasis - diagnosis Humans Immunochromatography - methods IMMUNODIAGNOSIS IMMUNOLOGICAL TECHNIQUES Infant Male Middle Aged Young Adult أمراض الإسهال أمراض المعدة و الأمعاء الإسهال التشخيص التشخيص المناعي الجياردية تقنيات التحصين علم الأمراض مسببات الأمراض مصر |
| title | Detection of Giardia Intestinalis Coproantigens in Diarrheic Samples by Immunochromatographic and Elisatechniques |
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