Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique
-Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can transform cells and dysregulate many cellular pathways involved in oncogenesis. This study was conducted to detect HCM...
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container_title | مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة. |
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creator | Abd Allah, Shadha F. Abd al-Majid, Ban Abbas Mukhlis, Fayizah A. |
description | -Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be
reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can
transform cells and dysregulate many cellular pathways involved in oncogenesis. This study
was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial
brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion
bodies in brain tumor tissues.
Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks
compared to eight normal brain autopsy specimens which were age and sex matched with the
study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA
Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.
Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed
hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens
representing (73.9% ) of the total study groups . All cases with high grades astrocytoma
revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 (
21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma
representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma
cases(5.3 %), revealed inclusion bodies on histological examination.
Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved
to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues.
Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular
studies are necessary to confirm the association of HCMV related human cancers in general Iraqi
population. |
format | Article |
fullrecord | <record><control><sourceid>emarefa</sourceid><recordid>TN_cdi_emarefa_primary_307928</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>307928</sourcerecordid><originalsourceid>FETCH-emarefa_primary_3079283</originalsourceid><addsrcrecordid>eNqFjE0KwjAUhIMoWLRHEN4FCmmLTbv2Bw_gwp281jR90CTapEI8vVXcu5qZb-CbsSgTvExEVl7mLEqrKk8ET4sli52jmmfFthJc8IjhXnrZeLIGbAvdqNFAE7zVUmFvnzSMDpQ00wYyoLEnZdB4UD1ZjQ7q8OGO_AhdqAe60Qu_tsnaGXqMcs0WLfZOxr9csc3xcN6dEqlxkC1e7wNNLVxzLqqszP_9b19cRNI</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>Abd Allah, Shadha F. ; Abd al-Majid, Ban Abbas ; Mukhlis, Fayizah A.</creator><creatorcontrib>Abd Allah, Shadha F. ; Abd al-Majid, Ban Abbas ; Mukhlis, Fayizah A.</creatorcontrib><description>-Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be
reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can
transform cells and dysregulate many cellular pathways involved in oncogenesis. This study
was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial
brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion
bodies in brain tumor tissues.
Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks
compared to eight normal brain autopsy specimens which were age and sex matched with the
study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA
Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.
Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed
hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens
representing (73.9% ) of the total study groups . All cases with high grades astrocytoma
revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 (
21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma
representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma
cases(5.3 %), revealed inclusion bodies on histological examination.
Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved
to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues.
Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular
studies are necessary to confirm the association of HCMV related human cancers in general Iraqi
population.</description><identifier>ISSN: 1993-7016</identifier><identifier>EISSN: 2708-728X</identifier><language>eng</language><publisher>النجف، العراق: جامعة الكوفة، مركز دراسات الكوفة</publisher><subject>Brain ; Cytomegalovirus infections ; Cytomegaloviruses ; Tumors ; الأورام ; الدماغ ; العقل</subject><ispartof>مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة., 2011, Vol.1 (22), p.181-186</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Abd Allah, Shadha F.</creatorcontrib><creatorcontrib>Abd al-Majid, Ban Abbas</creatorcontrib><creatorcontrib>Mukhlis, Fayizah A.</creatorcontrib><title>Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique</title><title>مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة.</title><description>-Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be
reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can
transform cells and dysregulate many cellular pathways involved in oncogenesis. This study
was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial
brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion
bodies in brain tumor tissues.
Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks
compared to eight normal brain autopsy specimens which were age and sex matched with the
study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA
Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.
Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed
hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens
representing (73.9% ) of the total study groups . All cases with high grades astrocytoma
revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 (
21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma
representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma
cases(5.3 %), revealed inclusion bodies on histological examination.
Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved
to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues.
Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular
studies are necessary to confirm the association of HCMV related human cancers in general Iraqi
population.</description><subject>Brain</subject><subject>Cytomegalovirus infections</subject><subject>Cytomegaloviruses</subject><subject>Tumors</subject><subject>الأورام</subject><subject>الدماغ</subject><subject>العقل</subject><issn>1993-7016</issn><issn>2708-728X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNqFjE0KwjAUhIMoWLRHEN4FCmmLTbv2Bw_gwp281jR90CTapEI8vVXcu5qZb-CbsSgTvExEVl7mLEqrKk8ET4sli52jmmfFthJc8IjhXnrZeLIGbAvdqNFAE7zVUmFvnzSMDpQ00wYyoLEnZdB4UD1ZjQ7q8OGO_AhdqAe60Qu_tsnaGXqMcs0WLfZOxr9csc3xcN6dEqlxkC1e7wNNLVxzLqqszP_9b19cRNI</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Abd Allah, Shadha F.</creator><creator>Abd al-Majid, Ban Abbas</creator><creator>Mukhlis, Fayizah A.</creator><general>جامعة الكوفة، مركز دراسات الكوفة</general><scope>ADJCN</scope><scope>AHFXO</scope></search><sort><creationdate>2011</creationdate><title>Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique</title><author>Abd Allah, Shadha F. ; Abd al-Majid, Ban Abbas ; Mukhlis, Fayizah A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-emarefa_primary_3079283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Brain</topic><topic>Cytomegalovirus infections</topic><topic>Cytomegaloviruses</topic><topic>Tumors</topic><topic>الأورام</topic><topic>الدماغ</topic><topic>العقل</topic><toplevel>online_resources</toplevel><creatorcontrib>Abd Allah, Shadha F.</creatorcontrib><creatorcontrib>Abd al-Majid, Ban Abbas</creatorcontrib><creatorcontrib>Mukhlis, Fayizah A.</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><jtitle>مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abd Allah, Shadha F.</au><au>Abd al-Majid, Ban Abbas</au><au>Mukhlis, Fayizah A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique</atitle><jtitle>مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة.</jtitle><date>2011</date><risdate>2011</risdate><volume>1</volume><issue>22</issue><spage>181</spage><epage>186</epage><pages>181-186</pages><issn>1993-7016</issn><eissn>2708-728X</eissn><abstract>-Background: Human Cytomegalovirus ( HCMV ), lies dormant in the glial cells , and can be
reactivated under conditions of inflammation and immunosuppression. In vitro, the virus can
transform cells and dysregulate many cellular pathways involved in oncogenesis. This study
was conducted to detect HCMV matrix-protein mRNA using In Situ Hybridization technique in glial
brain tumor tissues compared to normal brain tissues and the presence of cytomegalic inclusion
bodies in brain tumor tissues.
Patients and Method: Thirty eight of glial tumor specimens were obtained in paraffin blocks
compared to eight normal brain autopsy specimens which were age and sex matched with the
study group as a control group. ISH was conducted tissue sections using a biotinylated Long DNA
Probe for CMV Matrix Protein together with in-situ hybridization (ISH) detection kit.
Results: The biotinylated probe specific for mRNA encoded HCMV – Matrix Protein showed
hybridization with viral nucleic acids in 34 cases(out of 38)of malignant glial tumor specimens
representing (73.9% ) of the total study groups . All cases with high grades astrocytoma
revealed a positive hybridization in a percentage of 32.6% from 15 cases with grade III, and 10 (
21.7% ) cases with grade IV astrocytoma. Nine out of 38 cases with grade III astrocytoma
representing (23.7%), 7 (18.4 %) cases with glioblastoma multiforme and 2 oligodendroglioma
cases(5.3 %), revealed inclusion bodies on histological examination.
Conclusions: HCMV may play a role in the glioma pathogenesis. In Situ Hybridization test proved
to be a very sensitive and specific technique for the detection of HCMV mRNA in tissues.
Epidemiological, histopathological identification of cytomegalic inclusion bodies , and molecular
studies are necessary to confirm the association of HCMV related human cancers in general Iraqi
population.</abstract><cop>النجف، العراق</cop><pub>جامعة الكوفة، مركز دراسات الكوفة</pub><tpages>6</tpages></addata></record> |
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ispartof | مجلة مركز دراسات الكوفة : مجلة فصلية علمية محكمة., 2011, Vol.1 (22), p.181-186 |
issn | 1993-7016 2708-728X |
language | eng |
recordid | cdi_emarefa_primary_307928 |
source | EZB-FREE-00999 freely available EZB journals |
subjects | Brain Cytomegalovirus infections Cytomegaloviruses Tumors الأورام الدماغ العقل |
title | Detection of human cytomegalovirus genome in malignant gliomas by in situ hybridization technique |
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