Multi-center evaluation of one commercial and twelve in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Multi-center evaluation of one commercial and twelve in-house real-time PCR assays for detection of Mycoplasma pneumoniae

Abstract Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able...

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Veröffentlicht in:Diagnostic microbiology and infectious disease 2017
Hauptverfasser: Dumke, Roger, Benitez, Alvaro J, Chalker, Victoria, Gullsby, Karolina, Henrich, Birgit, Hidalgo-Grass, Carlos, Hoogenboezem, Theo, Kese, Darja, Loens, Katherine, Maaskant, Jolanda, Michael-Gayego, Ayelet, Moses, Allon E, Nir-Paz, Ran, Pas, Suzan D, Pereyre, Sabine, Petersen, Randi F, Rosenblatt, Meike, van Rossum, Annemarie M.C, Uldum, Søren A, Unger, Wendy W.J, Ursi, Dominique, Winchell, Jonas M, Bebear, Cecile
Format: Artikel
Sprache:eng
Schlagworte:
Infectious Disease
Internal Medicine
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Zusammenfassung:Abstract Detection of Mycoplasma pneumoniae by real-time PCR is not yet standardized across laboratories. We have implemented a standardization protocol to compare the performance of thirteen commercial and in-house approaches. Despite differences on threshold values of samples, all assays were able to detect at least 20 M. pneumoniae genomes per reaction.
ISSN:0732-8893
DOI:10.1016/j.diagmicrobio.2017.03.004