A Hin dIII Polymorphism of Fibronectin Gene Is Associated With Nephrolithiasis

Objectives To evaluate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis as a genetic risk factor. Methods Genomic DNA of 143 patients with calcium oxalate nephrolithiasis and 154 healthy controls were screened for polymorphisms ( Hae III b, Msp I, and Hin dIII) of the FN1 gene, using polymerase chain reaction-restriction fragments length polymorphism method. Allele and genotype frequencies were compared between the groups. Results Although the observed differences between distribution of genotypes of AA, AB, and BB (for Hae III b), as well as CC, CD, and DD ( Msp I) were not significant, FF genotype for Hin dIII showed significant difference when compared with both EF and EE + EF genotype ( P = .00202 and P = .00203, respectively). Conclusions The results of our study revealed that Hin dIII polymorphism of the FN1 gene is highly associated with calcium oxalate stone disease. This association makes FN a good candidate for further studies about the etiology of stone disease, and in the future it could be a candidate marker for evaluating the genetic risks in patients with nephrolithiasis.