Freeze Dried Platelets
A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then...
Gespeichert in:
| Hauptverfasser: | , , |
|---|---|
| Format: | Report |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | Spargo, Barry J Emler, Richard G Groel, Thomas R |
| description | A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then frozen at a temperature below the glass transition temperature of the suspension. A vacuum is placed on the frozen suspension to remove most of the water therefrom. Then, the temperature of the platelets is increased to the supercooled temperature while the vacuum is maintained. After being sealed under vacuum, the lyophilized platelets may be reconstituted to form viable, transfusable platelets. The reconstituted platelets have a high aggregation index, retain normal agglutination and degranulation capability, and are able to participate in clot formation. |
| format | Report |
| fullrecord | <record><control><sourceid>dtic_1RU</sourceid><recordid>TN_cdi_dtic_stinet_ADD017793</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ADD017793</sourcerecordid><originalsourceid>FETCH-dtic_stinet_ADD0177933</originalsourceid><addsrcrecordid>eNrjZBBzK0pNrUpVcCnKTE1RCMhJLEnNSS0p5mFgTUvMKU7lhdLcDDJuriHOHropJZnJ8cUlmXmpJfGOLi4GhubmlsbGBKQBOjcebA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>report</recordtype></control><display><type>report</type><title>Freeze Dried Platelets</title><source>DTIC Technical Reports</source><creator>Spargo, Barry J ; Emler, Richard G ; Groel, Thomas R</creator><creatorcontrib>Spargo, Barry J ; Emler, Richard G ; Groel, Thomas R ; DEPARTMENT OF THE NAVY WASHINGTON DC</creatorcontrib><description>A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then frozen at a temperature below the glass transition temperature of the suspension. A vacuum is placed on the frozen suspension to remove most of the water therefrom. Then, the temperature of the platelets is increased to the supercooled temperature while the vacuum is maintained. After being sealed under vacuum, the lyophilized platelets may be reconstituted to form viable, transfusable platelets. The reconstituted platelets have a high aggregation index, retain normal agglutination and degranulation capability, and are able to participate in clot formation.</description><language>eng</language><subject>AGGLUTINATION ; Biochemistry ; BLOOD PLATELETS ; BUFFERS(CHEMISTRY) ; COAGULATION ; FREEZE DRYING ; FREEZING ; GLASS ; LYOPHILIZATION ; Medicine and Medical Research ; PATENT APPLICATIONS ; POLYMERS ; SUPERCOOLING ; SUSPENSION DEVICES ; TEMPERATURE ; TRANSITION TEMPERATURE ; VACUUM ; WATER</subject><creationdate>1995</creationdate><rights>APPROVED FOR PUBLIC RELEASE</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,780,885,27567,27568</link.rule.ids><linktorsrc>$$Uhttps://apps.dtic.mil/sti/citations/ADD017793$$EView_record_in_DTIC$$FView_record_in_$$GDTIC$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>Spargo, Barry J</creatorcontrib><creatorcontrib>Emler, Richard G</creatorcontrib><creatorcontrib>Groel, Thomas R</creatorcontrib><creatorcontrib>DEPARTMENT OF THE NAVY WASHINGTON DC</creatorcontrib><title>Freeze Dried Platelets</title><description>A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then frozen at a temperature below the glass transition temperature of the suspension. A vacuum is placed on the frozen suspension to remove most of the water therefrom. Then, the temperature of the platelets is increased to the supercooled temperature while the vacuum is maintained. After being sealed under vacuum, the lyophilized platelets may be reconstituted to form viable, transfusable platelets. The reconstituted platelets have a high aggregation index, retain normal agglutination and degranulation capability, and are able to participate in clot formation.</description><subject>AGGLUTINATION</subject><subject>Biochemistry</subject><subject>BLOOD PLATELETS</subject><subject>BUFFERS(CHEMISTRY)</subject><subject>COAGULATION</subject><subject>FREEZE DRYING</subject><subject>FREEZING</subject><subject>GLASS</subject><subject>LYOPHILIZATION</subject><subject>Medicine and Medical Research</subject><subject>PATENT APPLICATIONS</subject><subject>POLYMERS</subject><subject>SUPERCOOLING</subject><subject>SUSPENSION DEVICES</subject><subject>TEMPERATURE</subject><subject>TRANSITION TEMPERATURE</subject><subject>VACUUM</subject><subject>WATER</subject><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>1995</creationdate><recordtype>report</recordtype><sourceid>1RU</sourceid><recordid>eNrjZBBzK0pNrUpVcCnKTE1RCMhJLEnNSS0p5mFgTUvMKU7lhdLcDDJuriHOHropJZnJ8cUlmXmpJfGOLi4GhubmlsbGBKQBOjcebA</recordid><startdate>19951020</startdate><enddate>19951020</enddate><creator>Spargo, Barry J</creator><creator>Emler, Richard G</creator><creator>Groel, Thomas R</creator><scope>1RU</scope><scope>BHM</scope></search><sort><creationdate>19951020</creationdate><title>Freeze Dried Platelets</title><author>Spargo, Barry J ; Emler, Richard G ; Groel, Thomas R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-dtic_stinet_ADD0177933</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>1995</creationdate><topic>AGGLUTINATION</topic><topic>Biochemistry</topic><topic>BLOOD PLATELETS</topic><topic>BUFFERS(CHEMISTRY)</topic><topic>COAGULATION</topic><topic>FREEZE DRYING</topic><topic>FREEZING</topic><topic>GLASS</topic><topic>LYOPHILIZATION</topic><topic>Medicine and Medical Research</topic><topic>PATENT APPLICATIONS</topic><topic>POLYMERS</topic><topic>SUPERCOOLING</topic><topic>SUSPENSION DEVICES</topic><topic>TEMPERATURE</topic><topic>TRANSITION TEMPERATURE</topic><topic>VACUUM</topic><topic>WATER</topic><toplevel>online_resources</toplevel><creatorcontrib>Spargo, Barry J</creatorcontrib><creatorcontrib>Emler, Richard G</creatorcontrib><creatorcontrib>Groel, Thomas R</creatorcontrib><creatorcontrib>DEPARTMENT OF THE NAVY WASHINGTON DC</creatorcontrib><collection>DTIC Technical Reports</collection><collection>DTIC STINET</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Spargo, Barry J</au><au>Emler, Richard G</au><au>Groel, Thomas R</au><aucorp>DEPARTMENT OF THE NAVY WASHINGTON DC</aucorp><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><btitle>Freeze Dried Platelets</btitle><date>1995-10-20</date><risdate>1995</risdate><abstract>A process and medium are disclosed for the lyophilization of platelets. During lyophilization, carbohydrate-load platelets are supercooled while suspended in a buffer solution including a biocompatible polymer that serves to preserve the structure of the platelets. The supercooled platelets are then frozen at a temperature below the glass transition temperature of the suspension. A vacuum is placed on the frozen suspension to remove most of the water therefrom. Then, the temperature of the platelets is increased to the supercooled temperature while the vacuum is maintained. After being sealed under vacuum, the lyophilized platelets may be reconstituted to form viable, transfusable platelets. The reconstituted platelets have a high aggregation index, retain normal agglutination and degranulation capability, and are able to participate in clot formation.</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | eng |
| recordid | cdi_dtic_stinet_ADD017793 |
| source | DTIC Technical Reports |
| subjects | AGGLUTINATION Biochemistry BLOOD PLATELETS BUFFERS(CHEMISTRY) COAGULATION FREEZE DRYING FREEZING GLASS LYOPHILIZATION Medicine and Medical Research PATENT APPLICATIONS POLYMERS SUPERCOOLING SUSPENSION DEVICES TEMPERATURE TRANSITION TEMPERATURE VACUUM WATER |
| title | Freeze Dried Platelets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T15%3A13%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-dtic_1RU&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.btitle=Freeze%20Dried%20Platelets&rft.au=Spargo,%20Barry%20J&rft.aucorp=DEPARTMENT%20OF%20THE%20NAVY%20WASHINGTON%20DC&rft.date=1995-10-20&rft_id=info:doi/&rft_dat=%3Cdtic_1RU%3EADD017793%3C/dtic_1RU%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |