Environmental Medicine Genome Bank (EMGB): Hardy-Weinberg Equilibrium at an Eotaxin Locus on Chromosome 17
The Environmental Medicine Genome Bank (EMGB) was used to test a low cost, high throughput, polymerase chain reaction (PCR) - based genetic strategy to distinguish 3 genotypes for a single nucleotide polymorphism (SNP) in the eotaxin gene located on chromosome 17. Using amplification refractory muta...
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| creator | Dikeidek, Murad Bastille, Amy Sonna, Larry A Lilly, Craig M |
| description | The Environmental Medicine Genome Bank (EMGB) was used to test a low cost, high throughput, polymerase chain reaction (PCR) - based genetic strategy to distinguish 3 genotypes for a single nucleotide polymorphism (SNP) in the eotaxin gene located on chromosome 17. Using amplification refractory mutation system PCR (ARMS-PCR), we determined the eotaxin ALA23-THR23 genotypes of 233 samples in the EMGB. The observed allele frequencies were then used to determine the distribution of genotypes that would be expected from the assumptions of the Hardy-Weinberg equilibrium. It was found that, for the overall cohort and for all but one small, heterogeneous subpopulation, the EMGB was in Hardy-Weinberg equilibrium at this locus. The EMGB can therefore serve as a useful source of control material for studies of genes located near this locus at cytogenetic position 17q21.
Prepared in cooperation with Brigham and Women's Hospital, Boston, MA. |
| format | Report |
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Prepared in cooperation with Brigham and Women's Hospital, Boston, MA.</description><subject>ASTHMA</subject><subject>CHAIN REACTIONS</subject><subject>CHROMOSOME 17</subject><subject>CHROMOSOMES</subject><subject>DEOXYRIBONUCLEIC ACIDS</subject><subject>DISEASES</subject><subject>EMGB(ENVIRONMENTAL MEDICINE GENOME BANK)</subject><subject>ENVIRONMENTAL DISEASES</subject><subject>ENVIRONMENTAL TESTS</subject><subject>EOTAXIN</subject><subject>GENES</subject><subject>Genetic Engineering and Molecular Biology</subject><subject>GENETICS</subject><subject>GENOTYPE</subject><subject>HARDY-WEINBERG EQUILIBRIUM</subject><subject>HETEROGENEITY</subject><subject>HIGH RATE</subject><subject>LOCUS</subject><subject>LOW COSTS</subject><subject>Medicine and Medical Research</subject><subject>NUCLEOTIDES</subject><subject>PCR(POLYMERASE CHAIN REACTION)</subject><subject>PERFORMANCE(HUMAN)</subject><subject>PHENOTYPE</subject><subject>POLYMERIZATION</subject><subject>POLYMORPHISM</subject><subject>SNP(SINGLE NUCLEOTIDE POLYMORPHISM)</subject><subject>WOUNDS AND INJURIES</subject><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>2002</creationdate><recordtype>report</recordtype><sourceid>1RU</sourceid><recordid>eNqFjTEOgkAQAGksjPoDiy21oDDEKHaAJxTSmViSBVZd5fbi3WH092pibzXFJDPD4KrkwdaIJvHYQUktNywEOYnRBCnKDWaqzNP5Bgq07Ss8EktN9gzq3nPHteVeA3pAAWU8Pllgb5regRHILtZo476lxWocDE7YOZr8OAqmO3XIirD13FTOf7a-SrZJFMfLaB390W_pVjv3</recordid><startdate>200201</startdate><enddate>200201</enddate><creator>Dikeidek, Murad</creator><creator>Bastille, Amy</creator><creator>Sonna, Larry A</creator><creator>Lilly, Craig M</creator><scope>1RU</scope><scope>BHM</scope></search><sort><creationdate>200201</creationdate><title>Environmental Medicine Genome Bank (EMGB): Hardy-Weinberg Equilibrium at an Eotaxin Locus on Chromosome 17</title><author>Dikeidek, Murad ; Bastille, Amy ; Sonna, Larry A ; Lilly, Craig M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-dtic_stinet_ADA3995383</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>2002</creationdate><topic>ASTHMA</topic><topic>CHAIN REACTIONS</topic><topic>CHROMOSOME 17</topic><topic>CHROMOSOMES</topic><topic>DEOXYRIBONUCLEIC ACIDS</topic><topic>DISEASES</topic><topic>EMGB(ENVIRONMENTAL MEDICINE GENOME BANK)</topic><topic>ENVIRONMENTAL DISEASES</topic><topic>ENVIRONMENTAL TESTS</topic><topic>EOTAXIN</topic><topic>GENES</topic><topic>Genetic Engineering and Molecular Biology</topic><topic>GENETICS</topic><topic>GENOTYPE</topic><topic>HARDY-WEINBERG EQUILIBRIUM</topic><topic>HETEROGENEITY</topic><topic>HIGH RATE</topic><topic>LOCUS</topic><topic>LOW COSTS</topic><topic>Medicine and Medical Research</topic><topic>NUCLEOTIDES</topic><topic>PCR(POLYMERASE CHAIN REACTION)</topic><topic>PERFORMANCE(HUMAN)</topic><topic>PHENOTYPE</topic><topic>POLYMERIZATION</topic><topic>POLYMORPHISM</topic><topic>SNP(SINGLE NUCLEOTIDE POLYMORPHISM)</topic><topic>WOUNDS AND INJURIES</topic><toplevel>online_resources</toplevel><creatorcontrib>Dikeidek, Murad</creatorcontrib><creatorcontrib>Bastille, Amy</creatorcontrib><creatorcontrib>Sonna, Larry A</creatorcontrib><creatorcontrib>Lilly, Craig M</creatorcontrib><creatorcontrib>ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA</creatorcontrib><collection>DTIC Technical Reports</collection><collection>DTIC STINET</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Dikeidek, Murad</au><au>Bastille, Amy</au><au>Sonna, Larry A</au><au>Lilly, Craig M</au><aucorp>ARMY RESEARCH INST OF ENVIRONMENTAL MEDICINE NATICK MA</aucorp><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><btitle>Environmental Medicine Genome Bank (EMGB): Hardy-Weinberg Equilibrium at an Eotaxin Locus on Chromosome 17</btitle><date>2002-01</date><risdate>2002</risdate><abstract>The Environmental Medicine Genome Bank (EMGB) was used to test a low cost, high throughput, polymerase chain reaction (PCR) - based genetic strategy to distinguish 3 genotypes for a single nucleotide polymorphism (SNP) in the eotaxin gene located on chromosome 17. Using amplification refractory mutation system PCR (ARMS-PCR), we determined the eotaxin ALA23-THR23 genotypes of 233 samples in the EMGB. The observed allele frequencies were then used to determine the distribution of genotypes that would be expected from the assumptions of the Hardy-Weinberg equilibrium. It was found that, for the overall cohort and for all but one small, heterogeneous subpopulation, the EMGB was in Hardy-Weinberg equilibrium at this locus. The EMGB can therefore serve as a useful source of control material for studies of genes located near this locus at cytogenetic position 17q21.
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| source | DTIC Technical Reports |
| subjects | ASTHMA CHAIN REACTIONS CHROMOSOME 17 CHROMOSOMES DEOXYRIBONUCLEIC ACIDS DISEASES EMGB(ENVIRONMENTAL MEDICINE GENOME BANK) ENVIRONMENTAL DISEASES ENVIRONMENTAL TESTS EOTAXIN GENES Genetic Engineering and Molecular Biology GENETICS GENOTYPE HARDY-WEINBERG EQUILIBRIUM HETEROGENEITY HIGH RATE LOCUS LOW COSTS Medicine and Medical Research NUCLEOTIDES PCR(POLYMERASE CHAIN REACTION) PERFORMANCE(HUMAN) PHENOTYPE POLYMERIZATION POLYMORPHISM SNP(SINGLE NUCLEOTIDE POLYMORPHISM) WOUNDS AND INJURIES |
| title | Environmental Medicine Genome Bank (EMGB): Hardy-Weinberg Equilibrium at an Eotaxin Locus on Chromosome 17 |
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