QUANTITATIVE DETERMINATION OF SOME CONSTITUENT LIPIDS IN HUMAN DENTIN

QUANTITATIVE DETERMINATION OF SOME CONSTITUENT LIPIDS IN HUMAN DENTIN

A method is described for the extraction of dentin lipids and the separation of these lipids into six components (cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids). Essentially, the method requires the decalcification of dentin using EDTA and subse...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Dirksen, Thomas R, Ikels, Kenneth G
Format: Report
Sprache:eng
Schlagworte:
Biochemistry
CALCIFICATION
CHOLESTEROL
DENTIN LIPIDS
DENTISTRY
GLYCERIDES
PHOSPHOLIPIDS
TEETH
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A method is described for the extraction of dentin lipids and the separation of these lipids into six components (cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids). Essentially, the method requires the decalcification of dentin using EDTA and subsequent extraction with diethyl ether, ethanol, and chloroform. The lipid extract is separated into six components by a small silicic acid column and the effluents are quantitated colorimetrically.