Microdetermination of cholesterol in serum lipoproteins

A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran...

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Veröffentlicht in:	Journal of lipid research 1977-03, Vol.18 (2), p.259-263
Hauptverfasser:	Heuck, C C, Nothhelfer, A, Raetzer, H, Schlierf, G
Format:	Artikel
Sprache:	eng
Schlagworte:	Cholesterol - analogs & derivatives Cholesterol Esters - blood Chromatography, Gas - methods Colorimetry - methods Evaluation Studies as Topic gasliquid chromatography Humans Hyperlipidemias - blood lipoprotein electrophoresis Lipoproteins - blood Lipoproteins, HDL - blood Lipoproteins, LDL - blood Lipoproteins, VLDL - blood Microchemistry Spectrophotometry - methods Ultracentrifugation - methods
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Beschreibung
Zusammenfassung:	A two-step procedure for the microdetermination of cholesterol in serum lipoproteins is compared with cholesterol quantitation after density gradient ultracentrifugation. Serum lipoproteins from 10 mul of serum are separated by electrophoresis on agarose and visualized by precipitation with dextran sulfate--CaCl2. The lipoprotein bands are cut off from the plates, the agarose slices are hydrolyzed by gas-liquid chromatography. The comparison between the two procedures reveals satisfactory correlations for beta-and pre-beta-lipoproteins and total serum. There is excellent recovery of cholesterol in fractionated lipoproteins.
ISSN:	0022-2275
DOI:	10.1016/S0022-2275(20)41705-5