N-Acetyl-L-cysteine Protects the Enterocyte against Oxidative Damage by Modulation of Mitochondrial Function

The neonatal small intestine is susceptible to damage caused by oxidative stress. This study aimed to evaluate the protective role of antioxidant N-acetylcysteine (NAC) in intestinal epithelial cells against oxidative damage induced by H2O2. IPEC-J2 cells were cultured in DMEM-H with NAC and H2O2. A...

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Veröffentlicht in:	Mediators of inflammation 2016-01, Vol.2016 (2016), p.1-9
Hauptverfasser:	Yin, Yulong, Huang, Bo, Guan, Guiping, Shao, Fangyuan, Wu, Miaomiao, Xiao, Hao, Tan, Bi’e
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylcysteine Acetylcysteine - pharmacology Animals Antioxidants - chemistry Apoptosis Apoptosis - drug effects bcl-X Protein - metabolism Bioenergetics Caspase 3 - metabolism Cell culture Cell growth Cell Proliferation Cell Survival Cytochrome Cytochromes c - metabolism Deoxyribonucleic acid DNA DNA - metabolism Enterocytes - drug effects Flow Cytometry Free Radical Scavengers - metabolism Gene expression Hogs Hydrogen Peroxide - chemistry Laboratories Mitochondria Mitochondria - drug effects Mitochondria - metabolism Oxidative Stress Physiological aspects Science Small intestine Studies Swine
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Zusammenfassung:	The neonatal small intestine is susceptible to damage caused by oxidative stress. This study aimed to evaluate the protective role of antioxidant N-acetylcysteine (NAC) in intestinal epithelial cells against oxidative damage induced by H2O2. IPEC-J2 cells were cultured in DMEM-H with NAC and H2O2. After 2-day incubation, IPEC-J2 cells were collected for analysis of DNA synthesis, antioxidation capacity, mitochondrial respiration, and cell apoptosis. The results showed that H2O2 significantly decreased (P
ISSN:	0962-9351 1466-1861
DOI:	10.1155/2016/8364279