Multi-Mode Compact Microscopy for High-Contrast and High-Resolution Imaging
We report a multi-mode compact microscope (MCM) for high-contrast and high-resolution imaging. The MCM consists of two LED illuminations, a magnification lens, a lift stage, and a housing with image processing and LED control boards. The MCM allows multi-modal imaging, including reflection, transmis...
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Veröffentlicht in: | Applied sciences 2022-08, Vol.12 (15), p.7399 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We report a multi-mode compact microscope (MCM) for high-contrast and high-resolution imaging. The MCM consists of two LED illuminations, a magnification lens, a lift stage, and a housing with image processing and LED control boards. The MCM allows multi-modal imaging, including reflection, transmission, and higher magnification modes. The dual illuminations also provide high-contrast imaging of various targets such as biological samples and microcircuits. The high dynamic range (HDR) imaging reconstruction of MCM increases the dynamic range of the acquired images by 1.36 times. The microlens array (MLA)-assisted MCM also improves image resolution through the magnified virtual image of MLA. The MLA-assisted MCM successfully provides a clear, magnified image by integrating a pinhole mask to prevent image overlap without additional alignment. The magnification of MLA-assisted MCM was increased by 3.92 times compared with that of MCM, and the higher magnification mode demonstrates the image resolution of 2.46 μm. The compact portable microscope can provide a new platform for defect inspection or disease detection on site. |
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ISSN: | 2076-3417 2076-3417 |
DOI: | 10.3390/app12157399 |