Spectroscopic Studies on the Interaction of Nano-TiO2 with Lysozyme

In the present study, the interaction between nano-TiO2 and lysozyme was investigated by the method of UV-Vis detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373) K in different buffer and pH, using temperature scanning spectroscopy. The presence of nano-TiO2 caused the destabilization of lysozyme resulting in a decrease in the temperature of unfolding with an increase in nano-TiO2 concentration.