MicroRNA profiling of ovarian granulosa cell tumours reveals novel diagnostic and prognostic markers

The aim of this study was to explore the clinical utility of microRNAs (miRNAs) as improved markers of ovarian granulosa cell tumours (GCTs) for cancer diagnosis and prognosis prediction. Current histopathological and genetic markers, such as the presence of a gene mutation to distinguish between th...

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Veröffentlicht in:	Clinical epigenetics 2017-07, Vol.9 (1), p.72-72, Article 72
Hauptverfasser:	Cheng, Wei-Tzu, Rosario, Roseanne, Muthukaruppan, Anita, Wilson, Michelle K, Payne, Kathryn, Fong, Peter C, Shelling, Andrew N, Blenkiron, Cherie
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Biomarkers Biomarkers, Tumor - genetics Cancer Care and treatment Cell growth Cell Line, Tumor Child Classification Diagnosis DNA Methylation Female Formaldehyde FOXL2 gene Gene expression Gene Expression Profiling Gene Expression Regulation, Neoplastic Gene mutation Genetic aspects Genetic markers Granulosa cell Granulosa Cell Tumor - diagnosis Granulosa Cell Tumor - genetics Granulosa Cell Tumor - pathology Gynecology Health aspects Humans Medical prognosis MicroRNA MicroRNAs MicroRNAs - genetics miRNA Mutation Neoplasm Recurrence, Local - genetics Neoplasm Staging Non-coding RNA Oligonucleotide Array Sequence Analysis - methods Ovarian cancer Ovarian tumors Paraffin Point mutation Polymerase chain reaction Prognosticator Sequence Analysis, RNA - methods Studies Surveillance Tissue biomarker Transcription factors Tumors
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Zusammenfassung:	The aim of this study was to explore the clinical utility of microRNAs (miRNAs) as improved markers of ovarian granulosa cell tumours (GCTs) for cancer diagnosis and prognosis prediction. Current histopathological and genetic markers, such as the presence of a gene mutation to distinguish between the two major subtypes are not wholly accurate and as such novel biomarkers are warranted. The miRNA expression profiles of five formalin-fixed, paraffin-embedded (FFPE) adult-GCTs and five juvenile-GCTs were assessed using Affymetrix miRNA 3.0 Arrays and compared for differential expression. Ten miRNAs were assessed in an additional 33 FFPE tumours and four normal granulosa cell samples by quantitative RT-PCR, and their expression correlated to clinical information. MicroRNA array found 37 miRNAs as differentially expressed between the two GCT subtypes (
ISSN:	1868-7075 1868-7083 1868-7083 1868-7075
DOI:	10.1186/s13148-017-0372-0