Transcriptome and proteome analysis of ultrasound pretreated peanut sprouts

•It was first time to combined transcriptomic and proteome analyses to investigate the ultrasound pretreated peanut sprouts.•A total of 1104 DEGs and 399 DEPs between ultrasound pretreated and nontreated peanut sprouts.•Ultrasound upregulated three key genes that could have increased the content of resveratrol via phenylpropanoid biosynthesis.•The genes and proteins related to phenylpropanoid biosynthesis, flavonoids biosynthesis, and lipid metabolism. Combined transcriptomic and proteome analyses were carried out to investigate the influence of ultrasound pretreatment on peanut sprouts. In total, 1104 differentially expressed genes (upregulated:538, downregulated:521) and 399 differentially accumulated proteins (upregulated: 197, downregulated: 202) were identified between ultrasound pretreated and nontreated peanut sprouts. These genes and proteins were related to a series of crucial biomolecular processes, including the metabolism of carbohydrates, terpenoids, and polyketides. The most enriched pathways were further analyzed in each category. Importantly, ultrasound upregulated three key genes namely the arahy. Tifrunner. gnm1.ann1.DXZI51, arahy.Tifrunner.gnm1.ann1.VGN2GE, and arahy.Tifrunner.gnm1.ann1.Y23DM6 that could have increased the content of resveratrol via phenylpropanoid biosynthesis. Furthermore, this study shows that B3, MYB transcription factor-like families play a significant role in response to ultrasound treatment. Overall, this study provides useful transcriptomics and proteomics information highlighting the molecular mechanisms that influence nutritional differences in peanut sprouts.