Biofilm removal effect of diatom complex on 3D printed denture base resin

For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is needed. The purpose of this study is to evaluate the efficacy of diatom complex with active micro-locomotion for removing biofilms...

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Veröffentlicht in:Scientific reports 2024-02, Vol.14 (1), p.4034-4034, Article 4034
Hauptverfasser: Choi, Sung-sil, Lee, Joo Hun, Kong, Hyunjoon, Park, Eun-Jin
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Sprache:eng
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Zusammenfassung:For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is needed. The purpose of this study is to evaluate the efficacy of diatom complex with active micro-locomotion for removing biofilms from 3D printed dentures. The diatom complex, which is made by doping MnO 2 nanosheets on diatom biosilica, is mixed with H 2 O 2 to generate fine air bubbles continuously. Denture base resin specimens were 3D printed in a roof shape, and Pseudomonas aeruginosa (10 7  CFU/mL) was cultured on those for biofilm formation. Cleaning solutions of phosphate-buffered saline (negative control, NC), 3% H 2 O 2 with peracetic acid (positive control, PC), denture cleanser tablet (DCT), 3% H 2 O 2 with 2 mg/mL diatom complex M (Melosira, DM), 3% H 2 O 2 with 2 mg/mL diatom complex A (Aulacoseira, DA), and DCT with 2 mg/mL DM were prepared and applied. To assess the efficacy of biofilm removal quantitatively, absorbance after cleaning was measured. To evaluate the stability of long-term use, surface roughness, ΔE, surface micro-hardness, and flexural strength of the 3D printed dentures were measured before and after cleaning. Cytotoxicity was evaluated using Cell Counting Kit-8. All statistical analyses were conducted using SPSS for Windows with one-way ANOVA, followed by Scheffe’s test as a post hoc ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-54408-y