Biofilm removal effect of diatom complex on 3D printed denture base resin
For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is needed. The purpose of this study is to evaluate the efficacy of diatom complex with active micro-locomotion for removing biofilms...
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Veröffentlicht in: | Scientific reports 2024-02, Vol.14 (1), p.4034-4034, Article 4034 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | For patients who have difficulty in mechanical cleaning of dental appliances, a denture cleaner that can remove biofilm with dense extracellular polymeric substances is needed. The purpose of this study is to evaluate the efficacy of diatom complex with active micro-locomotion for removing biofilms from 3D printed dentures. The diatom complex, which is made by doping MnO
2
nanosheets on diatom biosilica, is mixed with H
2
O
2
to generate fine air bubbles continuously. Denture base resin specimens were 3D printed in a roof shape, and
Pseudomonas aeruginosa
(10
7
CFU/mL) was cultured on those for biofilm formation. Cleaning solutions of phosphate-buffered saline (negative control, NC), 3% H
2
O
2
with peracetic acid (positive control, PC), denture cleanser tablet (DCT), 3% H
2
O
2
with 2 mg/mL diatom complex M (Melosira, DM), 3% H
2
O
2
with 2 mg/mL diatom complex A (Aulacoseira, DA), and DCT with 2 mg/mL DM were prepared and applied. To assess the efficacy of biofilm removal quantitatively, absorbance after cleaning was measured. To evaluate the stability of long-term use, surface roughness, ΔE, surface micro-hardness, and flexural strength of the 3D printed dentures were measured before and after cleaning. Cytotoxicity was evaluated using Cell Counting Kit-8. All statistical analyses were conducted using SPSS for Windows with one-way ANOVA, followed by Scheffe’s test as a post hoc (
p
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ISSN: | 2045-2322 2045-2322 |
DOI: | 10.1038/s41598-024-54408-y |