Antioxidant, Antiinflammation, and Antifibrotic Activity of Ciplukan ( Physalis angulata L). Extract

Linn. (Ciplukan) is a plant widely used in traditional medicine in subtropical and tropical regions. Most studies focus on its antioxidant and anti-inflammatory activity. Many studies also reported its therapeutic potential for treating cancer, malaria, hepatitis, rheumatism, liver problems, and tumors, but few studies have reported its anti-fibrosis activity. Here, we aimed to investigate the potential of as an antioxidant and anti-inflammatory that may be correlated with its anti-fibrosis action. In our study, we treated 3T3-L1 and TGF-β-induced 3T3-L1 cells with an ethanol extract of . We then monitored the cell's response, evaluated the antioxidant activity using an MTT assay, and observed the cells' migration using the cell scratch assay. We used RT-PCR to determine the expression of HIF-1α and IL-6 on TGF-β-induced 3T3-L1 cells. The ethanol extract of showed antioxidant activity and promoted cell proliferation on 3T3-L1 cells. Interestingly, the extract inhibited the migration of TGF-β-induced 3T3-L1 cells. Further analysis revealed that the extract could inhibit α expression and suppress expression on TGF-β-induced 3T3-L1 cells. The ethanol extract of showed antioxidant and anti-inflammation activities in 3T3-L1 cells. Both activities are associated with the antifibrotic activity of ethanol extract.