BNC1 promotes cell migration and invasion of lung squamous cell carcinoma

Objective To explore the mechanism and effects of Basonuclin 1(BNC1) on the migration and invasion abilities of lung squamous cell carcinoma. Methods Bioinformatics technology was used to analyze the expression patterns and survival impact of BNC1 in patients with lung squamous cell carcinoma in online databases; Tissue microarrays were stained by immunohistochemistry assay and analyzed for BNC1 protein level. Cell lines for lung squamous cell carcinoma with high and low BNC1 expression were screened in the Cancer Cell Line Encyclopedia (CCLE) database. Wound healing experiment and transwell assay were performed on lung squamous cell carcinoma cell models with knockdown and overexpression of BNC1, and the migration and invasion rates of each cell line were detected; Quantitative reverse transcription PCR(RT-qPCR) was used to validate the migration and invasion of 10 highly related genes derived from literature. Western blotting was used to validate the selected regulatory genes. Results Bioinformatics technol