Upper respiratory tract bacteria in Influenza-like illness cases in Indonesia using multiplex PCR method

Abstrak Latar belakang: Walaupun surveilans ILI di Indonesia sudah berlangsung sejak tahun 2006, namun belum diketahui data mengenai bakteri-bakteri yang dapat dideteksi dari kasus ILI dan kemungkinan menyebabkan ILI. Metode: Penelitian dilakukan pada bulan Maret – Desember tahun 2012, laboratorium Virologi Pusat Biomedis dan Teknologi Dasar Kesehatan menerima spesimen apus tenggorokan dan apus hidung dari 9 puskesmas yang menjadi sentinel surveilan ILI. Spesimen diperiksa menggunakan multipleks PCR untuk mendeteksi 6 panel bakteri. Hasil:Sebanyak 175 spesimen kasus ILI bakteri yang paling banyak ditemukan pada spesimen ini adalah Streptococcus pneumoniae (n=163), Haemophilus influenzae (n=146) dan Legionella pneumophila (41). Kesimpulan:Pada pasien ILI bakteri yang biasa terdeteksi pada saluran pernapasan bagian atas adalah Streptococcus pneumoniae, Haemophilus influenzae dan Legionella pneumophila. (Health Science Indones 2013;2:83-6)Kata kunci:infeksi bakteri, ILI, PCR multipleksAbstract Background: ILI surveillance in Indonesia has been conducted since 2006, but no data on the bacteria can be detected and caused ILI has been obtained. Method: From March to December 2012, Center for Research on Biomedic and Basic Health Technology’s laboratory was receiving throat and nose swab specimens from nine Public Health Centers appointed as the sentinels for ILI surveillance. These specimens were analyzed using multiplex PCR method, which detected six panels of bacteria. Results: 175 specimens taken from ILI patients contained three most frequently found species of bacteria: Streptococcus pneumoniae (n=163), Haemophilus influenzae (n=146) andLegionella pneumophila (41). Conclusion: In ILI patients, there are several bacteria most frequently detected in upper respiratory tract such as Streptococcus pneumoniae, Haemophilus influenzae and Legionella pneumophila. (Health Science Indonesia 2013;2:83-6)Key words:bacterial infection, ILI, multiplex PCR