Influence of nanosilver in hybrid carriers on morphological and biochemical blood pa-rameters of laying hens

The search for an alternative to antibiotics in poultry has led to a study of the effectiveness of using nanosilver preparations in the production of table eggs. The experiment determined the effect of the drug nanosilver in carriers based on polymer/inorganic hybrids (AgNPs/SPH) on morphological and biochemical parameters of the blood of laying hens. For this, 45 Hy-Line W36 hens were used at the age of 38 weeks, which were randomly divided into three groups. The AgNPs/SPH solution was administered 3 times a month with an interval of 10 days at concentrations of 0.0, 1.0, and 2.0 mg/L (0.0, 0.2 and 0.4 mg per hen per day). The introduction of AgNPs/SPH in doses of 0.2 and 0.4 mg per hen per day three times a month did not have a significant effect on the morphological parameters of the blood. A single dose of 0.2 mg AgNPs/SPH solution per hen per day increased the level of total protein, glucose, cholesterol, as well as the activity of alanine aminotransferase and alkaline phosphatase in the blood serum and decreased albumin, creatinine and gamma-glutamyl transpeptidase activity. Feeding laying hens a solution of nanosilver in a larger dose had a less pronounced effect on these indicators. Two-fold administration of AgNPs/SPH solution at a dose of 0.2 mg per laying hen per day increased only gamma-glutamyl transpeptidase activity in the blood serum, but decreased the level of total activity of protein, albumin, phosphorus, and alkaline phosphatase. At the same time, the drug nanosilver in double dose per day caused an increase in albumin content and alkaline phosphatase activity in the serum of hens. Triple feeding of laying hens with a solution of nanosilver at a dose of 0.2 mg per hen per day did not affect most of the biochemical parameters of serum, but in the double dose increased the content of total protein against the background of lowered cholesterol and gamma-glutamyl transpeptidase activity. With the increase in the frequency of feeding laying hens solutions of nanosilver in carriers based on polymer / inorganic hybrids, the level of severity of their impact on the metabolic profile of serum decreased. The results of research can be the basis for determining the optimal interval of application of nanosilver drugs in poultry, depending on the method of their synthesis and stabilization.