THE ANALYSIS OF CLINICAL, FUNCTIONAL, LABORATORY AND GENETIC PARAMETERS VALUES ON THE CONDITION OF PATIENTS AFTER MYOCARDIAL INFARCTION, AND PROGNOSIS OF INTRAMYOCARDIAL AUTOLOGIC CELL THERAPY EFFECTIVENESS FOR CHRONIC HEART FAILURE

Aim. To reveal or rule out an interconnection of the specifics of structure of clinical, functional, laboratory and genetic parameters of patients, who had myocardial infarction, with the effectiveness of intramyocardial autologic cell therapy (IACT).Material and methods. The study involved 71 patients, 87% of men, with ischemic heart disease and III-IV functional class (NYHA) heart failure at the age 37-76 y.o. (mean age 57,0±0,9 y.o.). The procedure of mobilized mononucleares loading into the blood and clinical effectiveness assessment was done at FSBI "SRICP n.a. E. N. Meshalkin" HM RF (Novosibirsk). The parameters of ischemic anamnesis were studied, left ventricle ejection volume, level of TNF-a and GM-CSF in cell cultures of patients' blood, the amount of CD34+ before and after G-CSFstimulation. The mononucleotide polymorphism of promotor region of cytokine genes studied (TNF-308, TNF-238, TNF-863, IL1B-511, IL1B- 31, IL4-590, IL6-174, IL10-592, IL10-1082), of endothelial growth factor (VEGF+936, VEGF-2578) and of matrix metalloproteinases (MMP2- 1306, MMP3-1171, MMP9-1562).Results. Among 11 clinical and laboratory parameters, and 14 polymorphic gene areas, just for two genotypes of VEGF in polymorphic position +936 C/T there were significant (p=0,0134) differences revealed for patients groups with and without long-term clinical effect of IACT usage. Amount of information obtained from complex quantitative parameters and genotypic signs increases dramatically (J=4,28-5,25, p