Antifungal activity of macrofungi extracts on phytopathogenic fungal strains of genera Fusarium sp. and Alternaria sp
During the last decades, intensive application of synthetic fungicides in the agricultural crop protection practice caused growing concern for the existence of toxic chemical residues in food as well as in the whole environment. Instead of using synthetic fungicides, it is suggested that crop protec...
Gespeichert in:
Veröffentlicht in: | Zbornik Matice Srpske za Prirodne Nauke 2017, Vol.2017 (133), p.231-240 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | During the last decades, intensive application of synthetic fungicides in the
agricultural crop protection practice caused growing concern for the
existence of toxic chemical residues in food as well as in the whole
environment. Instead of using synthetic fungicides, it is suggested that
crop protection be carried out by using preparations based on compounds of
natural origin (secondary metabolites of plants or microorganisms, including
macrofungi from Basidiomycota) as biological control agents. The potential
of macrofungal species as biocontrol agents was analyzed in this
investigation of eight autochthonous species from different locations in
Serbia. Both the terricolous species: Coprinus comatus, Coprinellus
truncorum, Amanita strobiliformis, Hydnum repandum and the lignicolous
species: Flammulina velutipes, Stereum subtomentosum, Trametes versicolor
and Bjerkandera adusta were examined, with an aim to detect some novel
sources of antifungal agents. This study surveyed antifungal activity of
selected macrofungal extracts (MeOH, EtOH and CHCl3) against phytopathogenic
Fusarium and Alternaria strains isolated from garlic, soybean and rice: F.
proliferatum, F. verticillioides, F. proliferatum, F. graminearum and A.
padwickii. Microdilution method in 96 well microplates was applied for the
estimation of antifungal effects of macrofungi extracts in the range from
24.75 to 198.00 mg/ml and determination of minimal inhibitory (MIC) and
minimal fungicidal concentration (MFC). EtOH extract of mychorhizal species
H. repandum showed antifungal activity against all analyzed phytopathogenic
strains, with the strongest effect on Fusarium strains (MIC 24.75 mg/ml; MFC
24.75 mg/ml). Among others, MeOH extracts of S. subtomentosum and C.
micaceus showed similar effects while only B. adusta showed slight effect on
Fusarium strains (MIC 24.75-99.00 mg/ml; MFC 24.75-99.00 mg/ml) and none
effect on A. padwickii. The obtained results indicate the possibility of
using examined extracts as efficient antifungal agents and provide the basis
for the new formulations of biocontrol agents against phytopathogenic fungi
in the future.
nema |
---|---|
ISSN: | 0352-4906 2406-0828 |
DOI: | 10.2298/ZMSPN1733231S |