Studies on Phenolic Profile, Antioxidant and Anticancer Effects of Different Polar Parts of Ethanol Extract of Jinhua Tibetan Tea

The phenolic components, antioxidation, and anticancer effects of different polar parts of ethanol extract of Jinhua Tibetan tea were studied. Three polar fractions, i.e., ethyl acetate, n-butyl alcohol, and water fractions, of ethanol extract of Jinhua Tibetan tea were obtained via liquid-liquid extraction process. The contents of total polyphenols and total flavonoids of the three fractions and ethanol extract were determined using Folin-Ciocalteu and aluminum chloride-potassium acetate method, respectively. The contents of tea polyphenol monomers in all the parts were determined using high-performance liquid chromatography (HPLC) method. Their antioxidant activities of all the parts were tested and compared using DPPH radical scavenging, ABTS+ radical scavenging, and Fe2+ ion chelating methods. Their anti-proliferation effects on human cervical cancer HeLa cell were evaluated using cell counting Kit8. Results indicated that ethyl acetate fraction had the highest contents of total polyphenols and total flav